Team:TU Darmstadt/Materials

From 2012.igem.org

(Difference between revisions)

Latest revision as of 01:28, 27 September 2012

Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the OWW materials page.

Can't find what you seek? Try their excellent page.

Strains

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

Antarctic Phosphatase

DNA polymerases

DNA ligases

T4 DNA ligase

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Imidazole

Other chemicals

Reporters

Dyes and stains

DAPI

Fluorescent proteins

GFP

@@ Line 24: / Line 24: @@
 		    <li><a href="/Team:TU_Darmstadt/Protocols" title="Protocols">Protocols</a></li>
 			<li><a href="/Team:TU_Darmstadt/Materials" title="Materials">Materials</a></li>
-			<li><a href="/Team:TU_Darmstadt/Simulaton" title="Simulation">Simulation</a></li>
+			<li><a href="/Team:TU_Darmstadt/Modeling" title="Modeling">Modeling</a></li>
 		    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
 		    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
-		<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul>
+		<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li>
-		    <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li>
-		    <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li>
-		    <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li>
 		<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
 		    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
@@ Line 40: / Line 37: @@
 </html>
+<span style="font-size:200%;"><span style="color:#00689D;">Materials</span></span>
-== Materials ==
+This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.
-=== Media ===
-==== Defined Media ====
-==== Standard Media ====
-=== Antibiotics ===
+Can't find what you seek? Try their excellent page.
+== Strains ==
+*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5&alpha;]
+*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
+== Media ==
+=== Modified Media ===
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Bromthymol_blue LB Bromothymol blue]
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Tributyrin LB Tributyrin]
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP-Medium GFP-Medium]
+=== Standard Media ===
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB]
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB]
+*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOC SOC]
+== Antibiotics ==
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ampicillin Ampicillin]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Chloramphenicol Chloramphenicol]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Kanamycin Kanamycin]
+== Enzymes ==
+=== Phosphatases ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Antarctic_Phosphatase Antarctic Phosphatase]
+=== DNA polymerases ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion Phusion]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Taq_polymerase Taq polymerase]
+=== DNA ligases ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Ligase T4 DNA ligase]
+=== Restriction enzymes ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/BsaI BsaI]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EcoRI EcoRI]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI PstI]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SfiI SfiI]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
+== Electrophoresis ==
+=== Agarose gel electrophoresis ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer Agarose gel loading buffer]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue Bromphenol blue]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DNA_ladder DNA ladder]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ethidium_Bromide Ethidium Bromide]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TAE TAE]
+=== PAGE ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Coomassie_stain Coomassie stain]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destain]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
+== Buffers ==
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Alkaline_phosphate_buffer Alkaline phosphate buffer]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Blotting_buffer Blotting buffer]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NEB_buffers NEB buffers]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Quiagen_buffers Quiagen buffers]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Resuspension_buffer Resuspension buffer]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Tris Tris]
+== Acids and bases ==
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Imidazole Imidazole]
+== Other chemicals ==
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Arabinose Arabinose]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/BCIP BCIP]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EDTA EDTA]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NiCl2 NiCl<sub>2</sub>]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NBT NBT]
+* [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
+* [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]
+== Reporters ==
+=== Dyes and stains ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DAPI DAPI]
+=== Fluorescent proteins ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP GFP]
 === Enzymes ===
-==== DNA polymerases ====
-==== DNA ligases ====
-==== Restriction enzymes ====
-==== Other ====
-=== Proteases ===
-=== Electrophoresis ===
+== Plasmids ==
-==== Agarose gel electrophoresis ====
+* [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
-==== PAGE ====
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEST100 pEST100-Cut]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEX pEX-6His-tag]
+* [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
+* [http://partsregistry.org/Part:pSB1A2 pSB1A2]
+* [http://partsregistry.org/Part:pSB1C3 pSB1C3]
+* [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26bpNBDSM13 pET26b(+)pNBDSM13]
+* [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26b pET26b(+)]
-=== Buffers ===
+== Primers ==
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ara_RBS Ara RBS]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Primer_Degradation Primer Degradation]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA503 tctA503 BioBrick]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA503#tctA503_Pst1 tctA503 Pst1]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA505 tctA505 BioBrick]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA505#tctA505_Pst1 tctA505 Pst1]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162 tctB162 BioBrick]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162#tctB162_Pst1 tctB162 Pst1]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162#tctB162_RBS tctB162 RBS]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB197 tctB197 BioBrick]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB197#tctB197_RBS tctB197 RBS]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC tphC BioBrick]
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC#tphC_Pst1 tphC Pst1]
-=== Acids and bases ===
+== Miscellaneous ==
-=== Other common chemicals ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Equipment Equipment]
-=== Reporters ===
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Materials Materials]
-==== Dyes and stains ====
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Software Software]
-==== Fluorescent proteins ====
+* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Metabolism Materials from Team Metabolism]
-==== Enzymes ====
-=== Detergents ===
-=== Miscellaneous ===

Team:TU Darmstadt/Materials

From 2012.igem.org

Latest revision as of 01:28, 27 September 2012

Contents

Strains

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other chemicals

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Plasmids

Primers

Miscellaneous