Team:TU Darmstadt/Materials/PBS

PBS

About

PBS stands for phosphate buffered saline and is a isotonic buffer solution.

Procedure

For 1x PBS

1. Combine:
   • 8.18 g NaCl (140 mM)
   • 0.2 g KCl (2.7 mM)
   • 1.77 g Na₂HPO₄ (10 mM)
   • 0.24 g KH₂PO₄ (1.8 mM)
   • Add. 1L dH₂O
2. Adjust to pH 7.4

For 10x PBS

1. Combine the following:
   • 80 g NaCl
   • 2 g KCl
   • 14.4 g Na₂HPO₄ (dibasic anhydrous) OR 18.1 g Na₂HPO₄ · 2H₂O (dibasic dihydrate) OR 27.2 g Na₂HPO₄ · 7H₂O (dibasic heptahydrate)
   • 2.4 g KH₂PO₄ (monobasic anhydrous)
   • 800 mL distilled H₂O
2. Adjust pH to 7.4 with HCl
3. Add H₂O to 1L
4. Autoclave for 20 minutes on liquid cycle. Store at RT.^[1]

References

[1] http://openwetware.org/wiki/PBS