Team:TU Darmstadt/Materials

Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

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Contents 1 Strains 2 Media 2.1 Modified Media 2.2 Standard Media 3 Antibiotics 4 Enzymes 4.1 Phosphatases 4.2 DNA polymerases 4.3 DNA ligases 4.4 Restriction enzymes 5 Electrophoresis 5.1 Agarose gel electrophoresis 5.2 PAGE 6 Buffers 7 Acids and bases 8 Other chemicals 9 Reporters 9.1 Dyes and stains 9.2 Fluorescent proteins 9.3 Enzymes 10 Plasmids 11 Primers 12 Miscellaneous

Strains

• [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
• [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]

Media

Modified Media

• LB Bromothymol blue
• LB Tributyrin
• GFP-Medium

Standard Media

• DYT
• LB
• SOB
• SOC

Antibiotics

• Ampicillin
• Chloramphenicol
• Kanamycin

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Phusion
• Taq polymerase

DNA ligases

• T4 DNA ligase

Restriction enzymes

• BsaI
• EcoRI
• PstI
• SfiI
• SpeI
• XbaI

Electrophoresis

Agarose gel electrophoresis

• Agarose gel loading buffer
• Bromphenol blue
• DNA ladder
• Ethidium Bromide
• Roti^® SafeGelStain
• TAE

PAGE

• Coomassie stain
• Destain
• SDS-Page

Buffers

• Alkaline phosphate buffer
• Blotting buffer
• NEB buffers
• PBS
• Quiagen buffers
• Resuspension buffer
• Tris

Acids and bases

• Imidazole

Other chemicals

• Arabinose
• BCIP
• EDTA
• NiCl₂
• NBT
• [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
• [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]

Reporters

Dyes and stains

• DAPI

Fluorescent proteins

• GFP

Enzymes

Plasmids

• [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
• pEST100-Cut
• pEX-6His-tag
• [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
• [http://partsregistry.org/Part:pSB1A2 pSB1A2]
• [http://partsregistry.org/Part:pSB1C3 pSB1C3]
• [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
• pET26b(+)pNBDSM13
• [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
• pET26b(+)

Primers

• Ara RBS
• Primer Degradation
• tctA503 BioBrick
• tctA503 Pst1
• tctA505 BioBrick
• tctA505 Pst1
• tctB162 BioBrick
• tctB162 Pst1
• tctB162 RBS
• tctB197 BioBrick
• tctB197 RBS
• tphC BioBrick
• tphC Pst1

Miscellaneous

• Equipment
• Materials
• Software
• Materials from Team Metabolism