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Team:TU Darmstadt/Materials/Antarctic Phosphatase
From 2012.igem.org
Contents |
Antarctic Phosphatase
About
Antarctic phosphatase catalyzes the removal of 5´ phosphate groups from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.[1]
Working conditions
Buffer: 1X Antarctic Phosphatase buffer
Supplier
- NEB http://www.neb.com/nebecomm/products/productM0289.asp
References
[1] http://www.neb.com/nebecomm/products/productM0289.asp
