Team:TU Darmstadt/Materials/XbaI

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XbaI

About

XbaI is a endonucleasic restriction enzyme isolated from Xanthomonas badrii. It creates stick ends with 5' end overhang.

Working conditions

The recognition motive of XbaI is:[1]

5' TCTAGA --> 5'---T     CTAGA---3'
3' AGATCT --> 3'---AGATC     T---5'

Buffer: NEBuffer 2 + BSA

SpeI requires at least 1bp overhang on its cutting site (99% cleavage efficiency).[2]

Supplier

References

[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_T-Z#X

[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp