Team:TU Darmstadt/Materials/SfiI

From 2012.igem.org

Contents

SfiI

About

SfiI is a endonucleasic restriction enzyme isolated from Streptomyces fimbriatus. It creates stick ends with 3' end overhang.

Working conditions

The recognition motive of SfiI is:[1] 

5' GGCCN5GGCC --> 5' ---GGCCNNNN   NGGCC--- 3'
3' CCGGN5CCGG --> 3' ---CCGGN   NNNNCCGG--- 5'

Buffer: NEBuffer 4 + BSA

SfiI requires at least 4bp overhang on its cutting site (93% cleavage efficiency).[2]

Supplier

References

[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_S#Whole_list_navigation

[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp

Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials/SfiI"