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Team:TU Darmstadt/Materials/Kanamycin
From 2012.igem.org
Contents |
Kanamycin
About
Kanamycin is an aminoglycoside antibiotic that interacts with the 30S subunit of prokayotic ribosomes inducing mistranslations thus inhibiting translocation during protein synthesis.[1]
Stock
- Mix:
- 3 g kanamycin (75 mg/mL)
- ad. 40 mL dist. water
- sterile filtration
- aliquot in 1 mL stocks and store at -20°C
Stability
- Kanamycin is one of the few autoclavable antibiotics
- Culture plates can be stored for about 2-3 month at 4°C (as a batch in a bag or individually sealed with Parafilm)
References
[1] Pestka, S.: "The Use of Inhibitors in Studies on Protein Synthesis", Methods in Enzymology 30, pp.261-282, 1975
