Team:TU Darmstadt/Materials/EDTA

From 2012.igem.org

EDTA

EDTA stands for ethylene-diamine-tetraacetic acid. It chelates divalent cations and is therefore used in many buffers. Its relative EGTA has a higher affinity for calcium than for magnesium ions.[1]

Application

For 100mL EDTA buffer:

  1. Combine:
    • 29.2 g EDTA (Ethylenediaminetetraacetic acid)
    • ad. 100 mL
  2. adjust to pH 8.0

References

[1] http://www.chm.bris.ac.uk/motm/edta/edtah.htm

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