Team:TU Darmstadt/Materials/NEB buffers

Contents 1 NEB buffers 1.1 NEBuffer 1 (yellow) 1.2 NEBuffer 2 (blue) 1.3 NEBuffer 3 (red) 1.4 NEBuffer 4 (green) 1.5 T4 DNA Ligase Buffer 1.6 NEBuffer EcoRI 1.7 References

NEB buffers

Composition of NEBuffers (1x) as provided by their website ^[1]:

NEBuffer 1 (yellow)

• 10 mM Bis Tris Propane-HCl
• 10 mM MgCl₂
• 1 mM DTT (pH 7.0 at 25°C).

NEBuffer 2 (blue)

• 10 mM Tris-HCl
• 10 mM MgCl₂
• 50 mM NaCl
• 1 mM DTT (pH 7.9 at 25°C)

NEBuffer 3 (red)

• 50 mM Tris-HCl
• 10 mM MgCl₂
• 100 mM NaCl
• 1 mM DTT (pH 7.9 at 25°C)

NEBuffer 4 (green)

• 20 mM Tris-acetate
• 10 mM magnesium acetate
• 50 mM potassium acetate
• 1 mM DTT (pH 7.9 at 25°C)

T4 DNA Ligase Buffer

• 50 mM Tris-HCl
• 10 mM MgCl₂
• 1 mM ATP
• 10 mM Dithiothreitol (pH 7.5 at 25°C)

NEBuffer EcoRI

• 100 mM Tris-HCl
• 50 mM NaCl
• 10 mM MgCl₂
• 0.025 % Triton X-100 (pH 7.5 at 25°C)

References

• NEB http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp