Team:TU Darmstadt/Materials/tctB197

From 2012.igem.org

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB197]

Primers for the construction of a BioBrick containing the small subunit B2 of the tripartite tricarboxylate transporter family.

tctB197 BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgtctgattccttccaagatctggc
rev: gtttcttcctgcagcggccgctactagtattacagccaccccgtttcagtcag

tctB197 RBS

Primers for the insertion of the arabinose promotor RBS site based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101].

     5'                                                                           3'
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAA AGAGGGGAC AAtactagatgtctgattccttccaagatctggc
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttacagccaccccgtttcagtcag