Team:TU Darmstadt/Materials

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Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

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Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other chemicals

  • Arabinose
  • BCIP
  • EDTA
  • NiCl2
  • NBT
  • [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
  • [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Plasmids

  • [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
  • pEST100-Cut
  • pEX-6His-tag
  • [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
  • [http://partsregistry.org/Part:pSB1A2 pSB1A2]
  • [http://partsregistry.org/Part:pSB1C3 pSB1C3]
  • [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
  • pET26b(+)pNBDSM13
  • [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
  • pET26b(+)

Primers

Miscellaneous