Team:TU Darmstadt/Materials

From 2012.igem.org

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Can't find what you seek? Try their excellent page.
Can't find what you seek? Try their excellent page.
== Strains ==
== Strains ==
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DH5alpha DH5α]
+
*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/TOP10 TOP10]
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*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
== Media ==
== Media ==
=== Modified Media ===
=== Modified Media ===
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Bromthymol_blue LB Bromthymol blue]
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*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Bromthymol_blue LB Bromothymol blue]
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Tributyrin LB Tributyrin]
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Tributyrin LB Tributyrin]
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP-Medium GFP-Medium]
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP-Medium GFP-Medium]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
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=== Other ===
 
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== Proteases ==
 
== Electrophoresis ==
== Electrophoresis ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NiCl2 NiCl<sub>2</sub>]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NiCl2 NiCl<sub>2</sub>]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NBT NBT]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NBT NBT]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PET PET]
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* [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TPA TPA]
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* [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]
== Reporters ==
== Reporters ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26bpNBDSM13 pET26b(+)pNBDSM13]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26bpNBDSM13 pET26b(+)pNBDSM13]
* [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
* [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26b pET26b(+)]
== Primers ==
== Primers ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC tphC BioBrick]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC tphC BioBrick]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC#tphC_Pst1 tphC Pst1]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC#tphC_Pst1 tphC Pst1]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEX_FsC_His_SfiI_up pEX FsC His SfiI up]
 
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEX_FsC_stop_SfiI_lo pEX FsC Stop SfiI lo]
 
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== Detergents ==
 
== Miscellaneous ==
== Miscellaneous ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Materials Materials]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Materials Materials]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Software Software]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Software Software]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Metabolism Materials from Team Metabolism]

Latest revision as of 01:28, 27 September 2012

Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

Can't find what you seek? Try their excellent page.

Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other chemicals

  • Arabinose
  • BCIP
  • EDTA
  • NiCl2
  • NBT
  • [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
  • [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Plasmids

  • [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
  • pEST100-Cut
  • pEX-6His-tag
  • [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
  • [http://partsregistry.org/Part:pSB1A2 pSB1A2]
  • [http://partsregistry.org/Part:pSB1C3 pSB1C3]
  • [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
  • pET26b(+)pNBDSM13
  • [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
  • pET26b(+)

Primers

Miscellaneous

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