Team:TU Darmstadt/Materials

From 2012.igem.org

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    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
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<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul>
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<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li>   
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    <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li>
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    <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li>
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    <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li>   
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
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<span style="font-size:200%;"><span style="color:#00689D;">Materials</span></span>
<span style="font-size:200%;"><span style="color:#00689D;">Materials</span></span>
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This page contains detailed instructions on the preparation of all employed materials.
+
This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.
 +
 
 +
Can't find what you seek? Try their excellent page.
 +
== Strains ==
 +
*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5&alpha;]
 +
*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
== Media ==
== Media ==
-
=== Defined Media ===
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=== Modified Media ===
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Bromthymol_blue LB Bromothymol blue]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Tributyrin LB Tributyrin]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP-Medium GFP-Medium]
 +
 
=== Standard Media ===
=== Standard Media ===
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Chloramphenicol Chloramphenicol]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Chloramphenicol Chloramphenicol]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Kanamycin Kanamycin]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Kanamycin Kanamycin]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Spectinomycin Spectinomycin]
 
== Enzymes ==
== Enzymes ==
 +
=== Phosphatases ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Antarctic_Phosphatase Antarctic Phosphatase]
=== DNA polymerases ===
=== DNA polymerases ===
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion Phusion]
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion Phusion]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Polymerase T4 DNA Polymerase]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Taq_polymerase Taq polymerase]
=== DNA ligases ===
=== DNA ligases ===
-
... 2 Uhr ich geh ins Bett  '''Du Faulpelz'''
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Ligase T4 DNA ligase]
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+
=== Restriction enzymes ===
=== Restriction enzymes ===
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=== Other ===
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/BsaI BsaI]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EcoRI EcoRI]
-
== Proteases ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI PstI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SfiI SfiI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
== Electrophoresis ==
== Electrophoresis ==
=== Agarose gel electrophoresis ===
=== Agarose gel electrophoresis ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer Agarose gel loading buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue Bromphenol blue]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DNA_ladder DNA ladder]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ethidium_Bromide Ethidium Bromide]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TAE TAE]
 +
=== PAGE ===
=== PAGE ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Coomassie_stain Coomassie stain]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destain]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
== Buffers ==
== Buffers ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Alkaline_phosphate_buffer Alkaline phosphate buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Blotting_buffer Blotting buffer]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NEB_buffers NEB buffers]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Quiagen_buffers Quiagen buffers]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Resuspension_buffer Resuspension buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Tris Tris]
== Acids and bases ==
== Acids and bases ==
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== Other common chemicals ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Imidazole Imidazole]
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 +
== Other chemicals ==
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Arabinose Arabinose]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/BCIP BCIP]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EDTA EDTA]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NiCl2 NiCl<sub>2</sub>]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NBT NBT]
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* [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
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* [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]
 +
 
== Reporters ==
== Reporters ==
=== Dyes and stains ===
=== Dyes and stains ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DAPI DAPI]
=== Fluorescent proteins ===
=== Fluorescent proteins ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP GFP]
=== Enzymes ===
=== Enzymes ===
-
== Detergents ==
+
 
 +
== Plasmids ==
 +
* [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEST100 pEST100-Cut]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEX pEX-6His-tag]
 +
* [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
 +
* [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
* [http://partsregistry.org/Part:pSB1C3 pSB1C3]
 +
* [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26bpNBDSM13 pET26b(+)pNBDSM13]
 +
* [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26b pET26b(+)]
 +
 
 +
== Primers ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ara_RBS Ara RBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Primer_Degradation Primer Degradation]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA503 tctA503 BioBrick]
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* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA503#tctA503_Pst1 tctA503 Pst1]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA505 tctA505 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA505#tctA505_Pst1 tctA505 Pst1]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162 tctB162 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162#tctB162_Pst1 tctB162 Pst1]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162#tctB162_RBS tctB162 RBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB197 tctB197 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB197#tctB197_RBS tctB197 RBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC tphC BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC#tphC_Pst1 tphC Pst1]
 +
 
== Miscellaneous ==
== Miscellaneous ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Equipment Equipment]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Materials Materials]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Software Software]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Metabolism Materials from Team Metabolism]

Latest revision as of 01:28, 27 September 2012

Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

Can't find what you seek? Try their excellent page.

Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other chemicals

  • Arabinose
  • BCIP
  • EDTA
  • NiCl2
  • NBT
  • [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
  • [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Plasmids

  • [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
  • pEST100-Cut
  • pEX-6His-tag
  • [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
  • [http://partsregistry.org/Part:pSB1A2 pSB1A2]
  • [http://partsregistry.org/Part:pSB1C3 pSB1C3]
  • [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
  • pET26b(+)pNBDSM13
  • [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
  • pET26b(+)

Primers

Miscellaneous

Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials"