Team:TU Darmstadt/Materials

From 2012.igem.org

(Difference between revisions)
(→Miscellaneous)
 
(57 intermediate revisions not shown)
Line 27: Line 27:
    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
-
<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul>
+
<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li>   
-
    <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li>
+
-
    <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li>
+
-
    <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li>   
+
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
Line 42: Line 39:
<span style="font-size:200%;"><span style="color:#00689D;">Materials</span></span>
<span style="font-size:200%;"><span style="color:#00689D;">Materials</span></span>
-
This page contains detailed instructions on the preparation of all employed materials.
+
This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.
-
=== Media ===
+
Can't find what you seek? Try their excellent page.
-
==== Defined Media ====
+
== Strains ==
-
==== Standard Media ====
+
*[http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5&alpha;]
-
[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
+
*[http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
-
[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB]
+
-
=== Antibiotics ===
+
 +
== Media ==
 +
=== Modified Media ===
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Bromthymol_blue LB Bromothymol blue]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB_Tributyrin LB Tributyrin]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP-Medium GFP-Medium]
 +
 +
=== Standard Media ===
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/DYT DYT]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOB SOB]
 +
*[https://2012.igem.org/Team:TU_Darmstadt/Materials/SOC SOC]
 +
 +
== Antibiotics ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ampicillin Ampicillin]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Chloramphenicol Chloramphenicol]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Kanamycin Kanamycin]
 +
 +
== Enzymes ==
 +
=== Phosphatases ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Antarctic_Phosphatase Antarctic Phosphatase]
 +
=== DNA polymerases ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion Phusion]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Taq_polymerase Taq polymerase]
 +
=== DNA ligases ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Ligase T4 DNA ligase]
 +
=== Restriction enzymes ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/BsaI BsaI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EcoRI EcoRI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI PstI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SfiI SfiI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
 +
 +
== Electrophoresis ==
 +
=== Agarose gel electrophoresis ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer Agarose gel loading buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue Bromphenol blue]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DNA_ladder DNA ladder]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ethidium_Bromide Ethidium Bromide]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TAE TAE]
 +
 +
=== PAGE ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Coomassie_stain Coomassie stain]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destain]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
 +
 +
== Buffers ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Alkaline_phosphate_buffer Alkaline phosphate buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Blotting_buffer Blotting buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NEB_buffers NEB buffers]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Quiagen_buffers Quiagen buffers]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Resuspension_buffer Resuspension buffer]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Tris Tris]
 +
 +
== Acids and bases ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Imidazole Imidazole]
 +
 +
== Other chemicals ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Arabinose Arabinose]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/BCIP BCIP]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EDTA EDTA]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NiCl2 NiCl<sub>2</sub>]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NBT NBT]
 +
* [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
 +
* [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]
 +
 +
== Reporters ==
 +
=== Dyes and stains ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DAPI DAPI]
 +
=== Fluorescent proteins ===
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/GFP GFP]
=== Enzymes ===
=== Enzymes ===
-
==== DNA polymerases ====
 
-
==== DNA ligases ====
 
-
==== Restriction enzymes ====
 
-
==== Other ====
 
-
=== Proteases ===
 
-
=== Electrophoresis ===
+
== Plasmids ==
-
==== Agarose gel electrophoresis ====
+
* [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
-
==== PAGE ====
+
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEST100 pEST100-Cut]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pEX pEX-6His-tag]
 +
* [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
 +
* [http://partsregistry.org/Part:pSB1A2 pSB1A2]
 +
* [http://partsregistry.org/Part:pSB1C3 pSB1C3]
 +
* [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26bpNBDSM13 pET26b(+)pNBDSM13]
 +
* [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/pET26b pET26b(+)]
-
=== Buffers ===
+
== Primers ==
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ara_RBS Ara RBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Primer_Degradation Primer Degradation]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA503 tctA503 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA503#tctA503_Pst1 tctA503 Pst1]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA505 tctA505 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctA505#tctA505_Pst1 tctA505 Pst1]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162 tctB162 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162#tctB162_Pst1 tctB162 Pst1]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB162#tctB162_RBS tctB162 RBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB197 tctB197 BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tctB197#tctB197_RBS tctB197 RBS]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC tphC BioBrick]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/tphC#tphC_Pst1 tphC Pst1]
-
=== Acids and bases ===
+
== Miscellaneous ==
-
=== Other common chemicals ===
+
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Equipment Equipment]
-
=== Reporters ===
+
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Materials Materials]
-
==== Dyes and stains ====
+
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Software Software]
-
==== Fluorescent proteins ====
+
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Metabolism Materials from Team Metabolism]
-
==== Enzymes ====
+
-
=== Detergents ===
+
-
=== Miscellaneous ===
+

Latest revision as of 01:28, 27 September 2012

Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

Can't find what you seek? Try their excellent page.

Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other chemicals

  • Arabinose
  • BCIP
  • EDTA
  • NiCl2
  • NBT
  • [http://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]
  • [http://en.wikipedia.org/wiki/Terephthalic_acid TPA]

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Plasmids

  • [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
  • pEST100-Cut
  • pEX-6His-tag
  • [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
  • [http://partsregistry.org/Part:pSB1A2 pSB1A2]
  • [http://partsregistry.org/Part:pSB1C3 pSB1C3]
  • [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
  • pET26b(+)pNBDSM13
  • [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
  • pET26b(+)

Primers

Miscellaneous

Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials"