User contributions

From 2012.igem.org

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• 14:08, 26 September 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 1September2012 ‎ (top)
• 15:33, 25 September 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 1September2012 ‎
• 14:35, 25 September 2012 (diff | hist) Team:Groningen/pigmentproduction ‎
• 14:04, 25 September 2012 (diff | hist) Team:Groningen/pigmentproduction ‎
• 13:06, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 31August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom/Arjan Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI....")
• 13:03, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 30August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom/Arjan Preparing overnight cultures of the Fnr and s-box promotors in psac.")
• 13:02, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 29August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom/Emeraldo PCR sbox and Fnr promotor. Ligation of Fnr promotor and s-box promotor into Psac.")
• 13:00, 30 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 28August2012 ‎
• 13:00, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 28August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom/Arjan Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac. Result: Incubation temperature of the ligation was too ...")
• 12:54, 30 August 2012 (diff | hist) Team:Groningen/Notebook ‎ (→Wetwork)
• 12:51, 30 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 25August2012 ‎
• 12:50, 30 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 25August2012 ‎
• 12:48, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 25August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom Ligation of alsT 300, Fnr and s-box promotor to GFP and lycopene in Psac with a new protocol. Psac was digested with EcorI. Fnr, alsT 300 and s-box ...")
• 12:37, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 21August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom Restriction analysis of previous obtained alsT 150, 250, 300, 500 and Fnr combined to GFP with EcorI. Incubation time was two hours at 37 degrees ce...")
• 12:32, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 17August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP. Result: None of the pcr products contained the desired product. It is not sur...")
• 12:27, 30 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 16August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom Ligation of Psb1c3 with Fnr promotor combined to GFP. For the digestion of Psb1c3, 258.2 ng was used. For the digestion of Fnr promotor and GFP, 239...")
• 09:39, 16 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 15August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3. Result: Transformation in E.coli was succesfull. PC...")
• 09:30, 16 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 14August2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom Ligation of Fnr promotor + GFP in psb1c3. Ratio of ligation 1:1:1 and 2:1:1 Fnr:GFP:psb1c3. Result: Transformation in E.coli did not work.")
• 09:18, 16 August 2012 (diff | hist) Team:Groningen/Notebook ‎ (→Wetwork)
• 10:28, 13 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 10August2012 ‎
• 10:26, 13 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 10August2012 ‎
• 10:23, 13 August 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 10August2012 ‎ (Created page with "{{HeaderGroningen2012}}")
• 10:22, 13 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 9August2012 ‎
• 14:03, 9 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 8August2012 ‎
• 14:03, 9 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 8August2012 ‎
• 13:57, 9 August 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 6August2012 ‎
• 13:29, 23 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 19July2012 ‎
• 13:26, 23 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 18July2012 ‎
• 13:25, 23 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 17July2012 ‎
• 14:22, 11 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 11July2012 ‎
• 14:22, 11 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 10July2012 ‎
• 14:21, 11 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 11July2012 ‎
• 14:17, 11 July 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 11July2012 ‎ (Created page with "{{HeaderGroningen2012}}")
• 14:17, 11 July 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 10July2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom PCR AlsT promotor with primers 150, 250, 300, 500 bp from AlsT gene at 58 °C and 59 °C. Result: PCR products were obtained for all primers excep...")
• 14:13, 11 July 2012 (diff | hist) Team:Groningen/Notebook ‎ (→Wetwork)
• 14:10, 11 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 3July2012 ‎
• 10:36, 2 July 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 29June2012 ‎ (Created page with "{{HeaderGroningen2012}} Tom Cutting plasmid backbone (BBa_090403) with only Xba1, only Pst1 and both Xba1 and Pst1. Samples were loaded on an 0.8% agarose gel.")
• 10:34, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 28June2012 ‎
• 10:28, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 27June2012 ‎
• 09:56, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 27June2012 ‎
• 09:39, 2 July 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 28June2012 ‎ (Created page with "{{HeaderGroningen2012}}")
• 09:38, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 26June2012 ‎
• 09:38, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 25June2012 ‎
• 09:37, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 21June2012 ‎
• 09:36, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 27June2012 ‎
• 09:34, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 27June2012 ‎
• 09:33, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 27June2012 ‎
• 09:32, 2 July 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 26June2012 ‎
• 12:36, 26 June 2012 (diff | hist) N Team:Groningen/Notebook/Wetwork 26June2012 ‎ (Created page with "{{HeaderGroningen2012}} Plasmid issolation of BBa_J37034 (LuxI added to GFP) and BBa_R0062 (promotor of LuxR (PR)). Concentrations were low. Colonies containing LuxI and LuxR ...")
• 12:30, 26 June 2012 (diff | hist) Team:Groningen/Notebook/Wetwork 25June2012 ‎

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