Team:Groningen/Notebook/Wetwork 17August2012



PCRed the different promotors alsT 150, 250, 300, 500 and Fnr to GFP.

Result: None of the pcr products contained the desired product. It is not sure if this is due to wrong primers or not appropriate working taq polymerase.


Cut the pSac-Cm and purified terminator PCR product with EcoRI and HindIII. Cut pSac-Cm was run on the gel and cut at the correct cut size and purified from the gel. Ligation of cut pSac-Cm with cut terminator and then transformation of E.coli DH5alpha with the ligation product.

Plasmid isolation of the transformans

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