Team:TU Darmstadt/Materials

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Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

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Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • TOP10

Media

Modified Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Other

Proteases

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Acids and bases

Other chemicals

Reporters

Dyes and stains

Fluorescent proteins

Enzymes

Plasmids

  • [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
  • pEST100-Cut
  • pEX-6His-tag
  • [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
  • [http://partsregistry.org/Part:pSB1A2 pSB1A2]
  • [http://partsregistry.org/Part:pSB1C3 pSB1C3]
  • [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
  • pET26b(+)pNBDSM13
  • [http://ecoliwiki.net/colipedia/index.php/pET-16b pET16b(+)]
  • pET26b(+)

Primers

Detergents

Miscellaneous