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Team:Grenoble/Biology/Protocols/Transformation 2
From 2012.igem.org
Revision as of 10:16, 24 August 2012 by
Adeline millet
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iGEM Grenoble 2012
iGEM 2012
main page
Overview
Network details
Biological "AND" gate
Protocols
Notebook
June
July
August
September
Protocols
In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will).
Cell culture
Medium composition
LB Agar plates preparation
Glycerol stock preparation
BioBricks utilisation
BioBricks extraction
Chemically competent
E. coli
cells production
Transformation of
E. coli
competent cells (TSS method)
Transformation of
E. coli
competent cells (heat shock)
Molecular biological techniques
PCR using a GoTaq polymerase
PCR using a HF Phusion polymerase
Gibson Assembly
Restriction enzyme digest
Ligation
Miniprep
Gel migration
Gel electrophoresis
DNA extraction
Test techniques
"AND" gate test (control of pBAD expression by cAMP and arabinose)
Abstract
Overview
Main Results
Attributions
Overview
Signaling Module
Amplification Module
Entire System
Notebook
Overview
Network details
Biological "AND" gate
Protocols
Notebook
Introduction
Legislation
Risk assessment
Answers to iGEM's questions
Downloads
Overwiew
Meeting
Cost assessment
Biobrick Safety Sheet
Promoting synthetic biology
The Team
Students
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Instructors
Introduction
Scientific Center
Students Event
Activities
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