Restriction enzyme digestion of DNA

Goal

Enzymes

• XbaI
• SpeI
• PstI
• EcoRI

Protocol

1. Set up the water bath at 37°C.


2. In an eppendorf tube, introduce :
   2 µL of each restriction enzyme
   10 µL of NEB 2 buffer
   1 µL of Bovine Serum Albumin (BSA)
   variable volume of template DNA
   to 50 µL of water
   
   

   SAFETY AND USEFUL RECOMMANDATION

   Those products are not dangerous at first sight, but in case of spreading or contacts with any part of the body, it is recommended to wash this part and to dry it after. Use a different pipette tip for each sample. It is also important to close tubes after putting the different reagents. DNA is more sensitive to its environment while it is not circulared in a plasmid and introduced into a cell. Therefore, a spreading has very few chances to lead to any bad consequences.

   
   
3. Put the eppendorf tube into the water bath.


4. Incubate at 37°C for 15 minutes.