Protocols

Cell culture

• Medium composition
• LB Agar plates preparation
• Glycerol stock preparation

BioBricks utilisation

• BioBricks extraction
• Transformation of E. coli cells (TSS method)
• Chemically competent E. coli cells production
• Transformation of E. coli competent cells (heat shock)

Molecular biological techniques

• PCR using a GoTaq® polymerase
• PCR using a HF Phusion polymerase
• Gibson Assembly
• Restriction enzyme digestion of DNA
• Ligation
• Plasmid Miniprep
• Double mutant construction

Gel migration

• Gel electrophoresis
• Ethidium Bromide (EtBr) revelation
• DNA extraction from Agarose gel

Test techniques

• "AND" gate test (control of pBAD expression by cAMP and arabinose)