Team:Grenoble/Biology/Protocols/Medium

From 2012.igem.org

iGEM Grenoble 2012

Project

Medium composition


LB (Lysogeny Broth) medium


 Tryptone  10 g/L 
 Yeast extract   5 g/L
 NaCl  5 g/L

M9 medium

Add ingredients in the following order:

 H2O  396.2 mL 
 CaCl2 (1M)  50 µL
 MgSO4 (1M)  1 mL
 Salts (5X)  100 mL
 Vitamine B1 (1%)    250 µL


The composition for a 2X solution of the salts is the following:

 Amount for 1L (in g)   FW
 Na2HPO4 x 2H2O    13.8  141.96 
 KH2PO4  6.1  136.09 
 NaCl  1.0  58.44
 NH4Cl  4.1  53.49