Team:TU Darmstadt/Materials

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Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

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Contents 1 Media 1.1 Defined Media 1.2 Standard Media 2 Antibiotics 3 Enzymes 3.1 Phosphatases 3.2 DNA polymerases 3.3 DNA ligases 3.4 Restriction enzymes 3.5 Other 4 Proteases 5 Electrophoresis 5.1 Agarose gel electrophoresis 5.2 PAGE 6 Buffers 7 Acids and bases 8 Other chemicals 9 Reporters 9.1 Dyes and stains 9.2 Fluorescent proteins 9.3 Enzymes 10 Plasmids 11 Detergents 12 Miscellaneous

Media

Defined Media

Standard Media

• DYT
• LB
• SOB
• SOC

Antibiotics

• Ampicillin
• Chloramphenicol
• Kanamycin

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Phusion
• Taq polymerase

DNA ligases

• T4 DNA ligase

Restriction enzymes

• BsaI
• EcoRI
• PstI
• SpeI
• XbaI

Other

Proteases

Electrophoresis

Agarose gel electrophoresis

• Agarose gel loading buffer
• Bromphenol blue
• DNA ladder
• Ethidium Bromide
• Roti^® SafeGelStain
• TAE

PAGE

• Coomassie stain
• Destain
• SDS-Page

Buffers

• Alkaline phosphate buffer
• NEB buffers
• PBS
• Quiagen buffers
• Tris

Acids and bases

• Imidazole

Other chemicals

• Arabinose
• BCIP
• EDTA
• NiCl₂
• NBT

Reporters

Dyes and stains

• DAPI

Fluorescent proteins

• GFP

Enzymes

Plasmids

• [http://partsregistry.org/Part:BBa_J61002 BBa J61002]
• [http://www.iba-lifesciences.com/details/product/107.html pPR-IBA2]
• [http://partsregistry.org/Part:pSB1A2 pSB1A2]
• [http://partsregistry.org/Part:pSB1C3 pSB1C3]
• [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]

Detergents

Miscellaneous

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