Notebook: Week 8

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Aims of the Week

The Lab team are aiming to begin assembly of Ribosome Binding Site and Constitutive Promoter, which could then be used for a number of our modules. James and Leonard have also been working towards characterising salt tolerance, and aim to begin doing this by the end of the week. The protocol for this experiment has been decided, and so it is likely we will have some characterisation data by the end of the week. Rhiannon's aim was to finish setting up the wiki LabBook, and publish it on the wiki. The Rathenau Debate Deadline is approaching, and Erin plans to make the final touches before it is sent off. Carina also wants to confirm her plans for the architectural project and begin a diary documenting her designs.

Monday

Wet Lab. After the concern that the small size of the BBa_J23119 and BBa_B0034 insert means we cannot truly detect the presence of the correct insert (Ext 7.3), Rhiannon and Aurelija decided to investigate other ways of detecting it. This included setting up a 25bp ladder on 3.5% gel, but neither the 12bp insert (BBa_B0034) or the 35bp insert (BBa_J23119) could be detected. This may be due to the low concentration of these plasmids - and so we are considering using PCR to amplify them.

Meeting - wiki. Rhiannon met with many of the team members to discuss the design for the Lab Book page. Our aim was to make the work description sufficiently detailed that it could be easily reproduced by others in the future. At the same time, we risked making it too long to read. For this reason, we have a summary infographic for each class of experiments, which clearly illustrate the method and results. However, we have also contained all of the necessary information in a drop-down from this image. While the design will continue to evolve, we are currently content with it at present.