Team:University College London/Notebook/Week7

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Contents

Notebook: Week 7

Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16

Aims of the Week

This week, 6 of our team members of going to the CAM conference in Munich, where they will be presenting our project at the poster session. They will also meet with the LMU Munich iGEM team to discuss a collaboration. This has been a really exciting event for the team, in particular the modellers who are keen to collaborate, as we have been talking with the Munich team on skype for several weeks. Remaining in London are most of the lab team, who want to get our important biobricks transformed and commence 3A assembly. Rhiannon intends to finish updating the Research pages of the wiki; in particular she is working on the overview and design pages, as well as developing some research diagrams. Philipp also is aiming to get some wiki programming done, so we can start including some of the features we have designed. Yeping will be contacting the Southbank centre in order to discuss organising a Public Engagement event, and will continue discussing safety with London Hackspace regarding the DIYbio streetlab. Carina will be working on the physical model of our island for the Human Practice event at Southbank, where members of the public can themselves help construct the island using plastic. She also aims to have a more specified idea of the architectural side of the project after a meeting with the team on Friday.


Monday

Wet Lab - In the lab today, Leonard transformed the TetR promoter. Rhiannon and Yeping set up a restriction digest for KS4000, I13522, KI123003, and K398108. A gel revealed that all except K123003 had been successful. Rhiannon and Yeping also picked colonies from J23119, I750016, ,B0015, and B0034 for an overnight culture.
Meeting - Human Practice. Yeping and Martina met with Mike Hughes from Gureilla Sciences. He had useful suggestions of how to make DIYbio more engaging, and had some useful contacts that may be able to help us with speed debating. He also passed on some tips on how to engage/approach people on the street for South bank project.

Tuesday

Wet Lab - In the lab today, Leonard transformed the TetR promoter. Rhiannon and Yeping set up a restriction digest for KS4000, I13522, KI123003, and K398108. A gel revealed that all except K123003 had been successful. Rhiannon and Yeping also picked colonies from J23119, I750016, ,B0015, and B0034 for an overnight culture.
Meeting - Primers - Leonard and James met with our supervisor Darren to discuss our primer design before an order is set through. This proved very useful, and they have taken the necessary steps to redesign the primers before they are sent off.
Meeting - Skype to the rest of team and Munich Conference - we caught up with the rest of the team this afternoon, and were pleased to find our project had been received very positively at the poster presentation. We also caught up on the key targets for the next few weeks, to ensure members of the Lab Team continue progress with Human Practice, while the rest of the team is away.

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