Restriction enzyme digestion of DNA

Goal

Protocol

1. Set up the water bath at 37°C.


2. In an eppendorf tube, introduce :
   2 µL of each restriction enzyme
   10 µL of NEBuffer 2
   1 µL of Bovine Serum Albumin (BSA).
   variable volume of template DNA
   to 50 µL of water
   
   

   SAFETY AND USEFUL RECOMMANDATION

   Those products are not dangerous at first sight, but in case of spreading or in case of products on hands or on any parts on the body, it is recommended to wash this part and to dry it after. Be careful to use for each sample a different sterile cone. It is also important to well close tubes after putting the ingredient in. Moreover the DNA cannot resist until it is not incorporated in a plasmid and into a cell. Therefore there is no chance that there are any possible consequences because of a leakage in the environment.

   
   
3. Put the eppendorf tube into the water bath.


4. Incubate at 37°C for 15 minutes.