Team:NYMU-Taipei/ymiq3.html
From 2012.igem.org
Resistance of Cyanobacteria (Synechococcus SP. PCC 7002) to Sulfide compound
Synechococcus sp. PCC7002 was cultured in the medium A2, a modification of Stevens et al. (1973) on a rotary shaker (100 rpm). The initial concentration of cells is controlled to an OD730 of 0.1 in fresh medium. Different concentration of sodium sulfide is added, and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was put into experimental group. Cells are grown in the six-well-plates and monitored under OD730 every 24 hour.
DCMU concentration and cell growth
This experiment is similar to the second one of Synechococcus SP. PCC 7002 testing series. The main idea was to find the suitable DCMU concentration for Synechococcus SP. PCC 7942. As a matter of fact, both wild type and sqr expressing strain are used in the experiment.
Sulfide concentration and the growth of sqr expressing strain Synechococcus SP. PCC 7942
It was expected that SQR expressing strain and wild type counterpart would have different growth rate under the presence of sulfide compounds. Though sulfide is naturally toxic to Synechococcus SP. PCC 7942, the strain with sqr should be able to metabolize sulfide and therefore prosper. As the result, we analyze H2S amount to detect whether sqr gene work or not. Therefore, we perform Chemical microvolume turbidimetry method to detect H2S concentration (see Sulfur Oxide Terminator part)
Sulfide oxidation in Escherichia coli expressing sulfide-quinone reductase gene
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Sulfide as Energy Generator
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Sulfur Oxide Terminator
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Denitrifying Machine
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Cd+2 Collector
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Becoming Venusian