Conclusion & References

  1. We have successfully cloned zinTp into pSB1C3
  2. We have successfully cloned zinTp-GFP into pSB1C3
  3. We have characterized zinTp function. The expression of downstream gene will increase when added cadmium ion into LB medium, and the maximum transcription rate occurs around 60 min after added 200μM cadmium.
  4. We have successfully cloned mnt into pSB1C3
  5. We have successfully cloned smtA into pSB1C3
  6. We have characterized smtA function. When expressing smtA, it enhances E.coli tolerance to cadmium.


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