Notebook: Week 3

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Aims For This Week

This week we will be presenting our progress in all aspects of the project to our supervisors, but our key aim is to demonstrate that we have progressed sufficiently to begin construction.

Training - Today four of us (James Rutley, Bethan Wolfenden, Bouran Sohrabi, Philipp Boeing) had a training on how to make competent cells which was organized by one of our iGEM advisers - Alex. We completed one out of three training stages which was making minimal media and plating out our e.coli. This will lead onto the second stage (tomorrow) which is inoculation of colonies.

Meeting - we had construction/characterization meeting with our main supervisor Darren Nesbeth. The outcome of the meeting was finalization of five modules which now are aggregation, salt tolerance, degradation, buoyancy, containment (receptor module was included into aggregation module). Tac promoter was chosen for degradation and salt tolerance modules in case any toxicity is present.

This meeting made our wet lab plan more solid this will allow us to start working on our first module (aggregation) the following week.