Team:Valencia Biocampus/talking

THE PROCESS

The main objective of our project is to accomplish a verbal communication with our microorganisms. To do that, we need to establish the following process:

Our aim in this part of the project is to detect when the yeast starts to ferment. To do this, we are going to use two gene constructions. The first construction consists of the ADH2 promoter fused to the YAP1 protein coding sequence. In the second construction the TRR promoter is fused to the GFP (Green Fluorescence Protein) coding sequence.

• The basic life cycle of our biological agent is based on an input/output process through the use of interfaces.
• The input used is a voice signal (question), which will be collected by our voice recognizer
• The voice recognizer identifies the question and, through the program in charge of establishing the communication, its corresponding identifier is written in the assigned port of the arduino.
• The (sw=software?) of the arduino reads the written identifier and, according to it, the corresponding port is selected, indicating the flourimeter which wavelength has to be emitted on the culture. There are four possible questions (q), and each of them is associated to a different wavelength.
• The fluorimeter emits light (Bioinput), exciting the compound through optic filters.
• Due to the excitation produced, the compound emits fluorescence (BioOutput), which is measured by the fluorimeter with a sensor.
• This fluorescence corresponds to one of the four possible answers (r: response)
• The program of the arduino identifies the answer and writes its identifier in the corresponding port.
• The communication program reads the identifier of the answer from the port.
• Julius emits the answer via a voice signal. (Output)