Meeting – What is left to design? We met early to discuss issues remaining in our project. Our particular focus was to find a means to prevent plasmids from our bacteria entering the marine ecosystem (Containment Module). Bacterial conjugation – the key means of exchanging DNA – is our primary target, but minor forms must also be included.

Meeting – Reviewing Biobrick Construction. The team met with our supervisor Darren Nesbeth for further discussion of biobrick construction and, importantly, to narrow down the selection of names for our project. A vote identified three popular names – Plastic Republic, Syntopia and Biotopia. A poll was set up on our facebook to see which was most popular with our facebook followers.

Press Team Meeting – What Should a Press Kit Contain? The team met to discuss how we can convey our project to the Press – in particular whether it would be best to place the focus on the problem of microplastics, or the concept of an island as a solution for this problem. For the press release, and for our other forms of media, we have decided to focus on the island. Though an ‘island’ it is an illusory endpoint to our project – it is highly visible, and will foo idea that synthetic biology can be used to improve the state of the planet.

Construction Crew Meeting – Conjugation. The Construction Crew continued their search for a way to prevent conjugation. They discussed ‘geneageddon’ - a theoretical biobrick enabling bacteria to secrete nucleases, which will digest any plasmids released during cell lysis. Also, they considered using the Imperial iGEM 2011 toxin/antitoxin system to prevent gene transfer by conjugation. Our GMO would carry a toxin gene on its plasmids and an antitoxin gene on its chromosomal DNA. Upon conjugation, only the toxin gene would be delivered to the wild-type recipient, and so any conjugation between GMO and wild-type would be lethal for the wild-type. Spread of genetically modified DNA would therefore be prevented. In particular, we aim to combine these genes on a single plasmid, and therefore improve the Imperial biobrick.

Modellers meeting - Using models to inform experimental design. This afternoon there was a meeting between computer Modellers, Advisors, and the Construction Crew to discuss specific variables required for modelling our experiments.

Wiki meeting – implementing the wiki. Prior to this meeting, the Wiki Team had discussed ways in which to make the wiki coherent, and had developed a number of new ideas and designed a number of layouts. This evening, we began writing the content of the wiki, and the process of creating the site.

Meeting -Construction. The Construction Crew worked hard all afternoon to produce a final document of all the biobrick circuits.