Team:NYMU-Taipei/ymiq2.html

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   <p><span class="subtitle">Resistance of Cyanobacteria to Sulfide compound --&gt; Sulfide-Quinone Reductase</span></p>
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   <p><span class="subtitle">Resistance of Synechococcus SP. PCC 7002 to 3 - (3,4-dichlorophenyl) - 1,1 – dimethylurea (DCMU)</span></p>
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   <p>Several Cyanobacteria have Sulfide-Quinone Reductase (SQR) and thus the ability to deprive electron from sulfide compound. According to both databases of NCBI and KEGG, the sqr in Synechococcus SP. PCC 7002 shared great similarity with that of Oscillatoria limnetica, which is reported to exhibit anoxygenic photosynthesis by consumed sulfide anion. Since we planned to express sqr from Synechococcus SP. PCC 7002 in Synechococcus SP. PCC 7942 and Escherichia coli, the experiment was designed to testify the property of the sqr. DCMU was added in the medium to inhibit photosystem II, and therefore only sodium sulfide in the medium can provide electron for carbon photoassimilation. By creating different dilution of sodium sulfide, we expected that the more sodium sulfide was present, the better the cell grew. <br />
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   <p>From the previous research, we discovered that the concentration of 3 - (3,4 - dichlorophenyl) - 1,1 – dimethylurea (DCMU) must be adjusted to meet our requirement. Under certain DCMU concentration, the presence of sulfide would be extreme decisive condition which determines whether the colonies live or die. In this experiment, DCMU is diluted with A2 medium to explore the relationship between DCMU concentration and cell growth. Sodium sulfide is added to the experimental group and its initial concentration is controlled to 10 mM. <br />
    
    
    
    
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    DCMU structure and its mechanism on photosynthesis<br />
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    The special &rdquo;CO2&rdquo; infused  device for cyanobacteria incubation<br />
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  <p><span class="subtitle">Sodium sulfide concentration and cell growth</span></p>
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    <p>From the previous  studies, it is suggested that <em>Synechococcus  SP. PCC 7002 </em>is able to metabolize sulfide compounds. We took advantage of  the results in our last experiment and adjusted the concentration of DCMU to an  appropriate degree. Since sulfide would become the main reducing energy for photoassimilation  under the effect of DCMU, we believe the more sulfide concentration in the  wells, the better cell growth would be observed.</p>
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      <div class=out style='text-align:center'><span class="out" style="text-align:center"><img src="images/ymiq4.png" width="573" height="278" border="0" align="center"  /></span><br />
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Revision as of 09:42, 18 October 2012

NYMU iGEM

Methods

Resistance of Synechococcus SP. PCC 7002 to 3 - (3,4-dichlorophenyl) - 1,1 – dimethylurea (DCMU)

From the previous research, we discovered that the concentration of 3 - (3,4 - dichlorophenyl) - 1,1 – dimethylurea (DCMU) must be adjusted to meet our requirement. Under certain DCMU concentration, the presence of sulfide would be extreme decisive condition which determines whether the colonies live or die. In this experiment, DCMU is diluted with A2 medium to explore the relationship between DCMU concentration and cell growth. Sodium sulfide is added to the experimental group and its initial concentration is controlled to 10 mM.


DCMU structure and its mechanism on photosynthesis
http://en.wikipedia.org/wiki/File:Diuron.png

Sodium sulfide concentration and cell growth

From the previous studies, it is suggested that Synechococcus SP. PCC 7002 is able to metabolize sulfide compounds. We took advantage of the results in our last experiment and adjusted the concentration of DCMU to an appropriate degree. Since sulfide would become the main reducing energy for photoassimilation under the effect of DCMU, we believe the more sulfide concentration in the wells, the better cell growth would be observed.


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