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Team:Calgary/Notebook/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 29: | Line 29: | ||
| - | <h2>Desulfurization</h2> | + | <h2>Desulfurization Protocols</h2> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/catalase">Catalase assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/catalase">Catalase assay</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/desulfur">Desulfurization Assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/desulfur">Desulfurization Assay</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hpac">HpaC assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hpac">HpaC assay</a></li> | ||
| - | <h2>Decarboxylation</h2> | + | <h2>Decarboxylation Protocols</h2> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/PetroBrick Validation Assay">PetroBrick Validation Assay </a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/PetroBrick Validation Assay">PetroBrick Validation Assay </a></li> | ||
| - | <h2>Denitrogenation</h2> | + | <h2>Denitrogenation Protocols</h2> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oextraction">Organic Extraction</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oextraction">Organic Extraction</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/carbazole">Carbazole Degradation Assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/carbazole">Carbazole Degradation Assay</a></li> | ||
| Line 43: | Line 43: | ||
| - | <h2>Decatecholization</h2> | + | <h2>Decatecholization Protocols</h2> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization Assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization Assay</a></li> | ||
<h2>Transposon Mutant Library for Toxin Detection</h2> | <h2>Transposon Mutant Library for Toxin Detection</h2> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li> | ||
| - | <h2>Kill Switch</h2> | + | <h2>Kill Switch Protocols</h2> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">Characterization of mgtA regulation with GFP </a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">Characterization of mgtA regulation with GFP </a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit">Characterization of mgtA regulation with S7 killgene </a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit">Characterization of mgtA regulation with S7 killgene </a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/nucleaseassay">Nuclease assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/nucleaseassay">Nuclease assay</a></li> | ||
| - | <h2>Bioreactor Assays</h2> | + | <h2>Bioreactor Assays Protocols</h2> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/bacgrowth">Bacterial Growth Experiments</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/bacgrowth">Bacterial Growth Experiments</a></li> | ||
<p>Belt Selection Tests:</p> | <p>Belt Selection Tests:</p> | ||
Revision as of 22:36, 3 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- Gel Extraction
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DMSZ
- Site-Directed Mutagenesis
- Taq PCR Protocol
Electrochemistry Protocols
- Cyclic Voltammetry
- Potentiostatic Standard Curve Generation
- Reporter Expression Detection
Desulfurization Protocols
- Catalase assay
- Desulfurization Assay
- HpaC assay
Decarboxylation Protocols
- PetroBrick Validation Assay
Denitrogenation Protocols
- Organic Extraction
- Carbazole Degradation Assay
- GC-MS Analysis
Decatecholization Protocols
- Decatecholization Assay
Transposon Mutant Library for Toxin Detection
- Transposon-Mediated Mutant Library Generation
Kill Switch Protocols
- Characterization of mgtA regulation with GFP
- Characterization of mgtA regulation with S7 killgene
- Nuclease assay
Bioreactor Assays Protocols
- Bacterial Growth Experiments
Belt Selection Tests:
- Hydrocarbon Test
- Tailings Test
- Bacteria Test
- Hydrocarbon Separation Test
- NA and Hexadecane Belt Skim Test
Modeling
- Modelling validation experiments
