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Team:Calgary/Notebook/Protocols
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- GC-MS Analysis
- Gel Extraction
- Gibson Assembly
- LB Agar Plates
- M9 Minimal Media Preparation
- Organic Extraction
- Overnight Cultures
- PCR Purification
- PicoGreen Assay
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DSMZ
- Site-Directed Mutagenesis
- Splice Overlap Extension PCR (SOE PCR)
Electrochemistry Protocols
- Cyclic Voltammetry
- Potentiostatic Standard Curve Generation
- Reporter Expression Detection
Desulfurization Protocols
- Catalase assay
- Desulfurization Assay
- HpaC assay
Decarboxylation Protocols
- PetroBrick Validation Assay
- oleT Validation Assay
Denitrogenation Protocols
- AmdA Characterization Assay
- Carbazole Degradation Assay
Decatecholization Protocols
- Decatecholization Assay
Transposon Mutant Library for Toxin Detection
- Transposon-Mediated Mutant Library Generation
Kill Switch Protocols
- Characterization of mgtA regulation with GFP
- Characterization of mgtA regulation with S7 killgene
- Characterization of Prha with GFP
- Kill Assay for rhamnose system
- Glycine Auxotrophic Assays (Glycine Media Test, and Petrobrick Test)
- Nuclease assay
- Bacterial Growth Experiments
- Bacteria Test
- Hydrocarbon Separation Test
- Hydrocarbon Test
- NA and Hexadecane Belt Skim Test
- Tailings Test
- Modelling validation experiments
General Protocols
Bioreactor Assays Protocols
Belt Selection Tests:
