Characterization of mgtA regulation with GFP

1. Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl2.
• mgtAp-rb-K082003
• mgtAp-rb
• R0040-mgtArb-K082003
• R0040-B0034-K082003
2. The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl
   • 0mM MgCl₂
   • 0.01mM MgCl₂
   • 0.1mM MgCl₂
   • 1mM MgCl₂
   • 10mM MgCl₂
3. Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.