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Team:Calgary/Notebook/Protocols
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Revision as of 09:55, 3 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- Gel Extraction
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DMSZ
- Site-Directed Mutagenesis
- Taq PCR Protocol
Electrochemistry Protocols
- Electorchemistry protocols (include agarose bead making)
Desulfurization
- Desulfurization Assay
- Catalase assay
- HPAC assay
Decarboxylation
- PetroBrick Validation Assay
Denitrogenation
- Organic Extraction
- Carbzazole Degradation Assay
- GC-MS Analysis
Decatecholization
- Decatecholization (xylE and incubation with the Petrobrick)
Transposon Mutant Library for Toxin Detection
- Transposon-Mediated Mutant Library Generation
Kill Switch
- Nuclease assay
- Magnesium circuit characterization
- Characterization of the mgtA circuit with S7 promoter
Bioreactor Assays
Belt Selection Tests:
- Hydrocarbon Test
- Tailings Test
- Bacteria Test
- Hydrocarbon Separation Test
- NA and Hexadecane Belt Skim Test
Modeling
- Modelling validation experiments
