Revision as of 07:06, 3 October 2012

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Protocols

Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.

General Protocols

Agarose Gel Electrophresis
Bacterial Genomic DNA Purification
Bacterial Transformation
Construction Techniques
Gel Extraction
LB Agar Plates
Overnight Cultures
PCR Purification
Plasmid Purification (from E. coli)
Preparing Chemically Competent Cells (E. coli)
Preparing Glycerol Stocks (E. coli)
Rehydration of Registry DNA
Reviving Freeze-dried Bacterial Cultures from DMSZ
Site-Directed Mutagenesis
Taq PCR Protocol

Electrochemistry Protocols

Electorchemistry protocols (include agarose bead making)

Desulfurization

Decarboxylation

Petrobrick/oleT assay protocols/ GC MS

Denitrogenation

Organic Extraction
Carbzazole Degradation Assay

Decatecholization

Decatecholization (xylE and incubation with the Petrobrick)

Transposon Mutant Library for Toxin Detection

Transposon-Mediated Mutant Library Generation

Kill Switch

Nuclease assay
Magnesium circuit characterization
Characterization of the mgtA circuit with S7 promoter

Bioreactor

Bioreactor assays

Modeling

Modelling validation experiments

@@ Line 42: / Line 42: @@
 <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization (xylE and incubation with the Petrobrick)</a></li>
-<h2>Transposon</h2>
+<h2>Transposon Mutant Library for Toxin Detection</h2>
+<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li>
 <h2>Kill Switch</h2>