"
Team:Calgary/Notebook/Protocols/mgcircuit
From 2012.igem.org
(Difference between revisions)
(Created page with "{{Team:Calgary/TemplateNotebookOrange| TITLE=Characterization of the magnesium circuit| CONTENT=<html> <ol> <li>Cells with the appropriate circuits were grown in LB overnight wit...") |
|||
| Line 8: | Line 8: | ||
</ol> | </ol> | ||
| - | + | <br><br><br><br><br><br><br><br><br><br> | |
</html>}} | </html>}} | ||
Revision as of 03:35, 2 October 2012
Hello! iGEM Calgary's wiki functions best with Javascript enabled, especially for mobile devices. We recommend that you enable Javascript on your device for the best wiki-viewing experience. Thanks!
Characterization of the magnesium circuit
- Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.
