Team:University College London/Notebook/Week9
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<div class="notebook-achievement"> '''Achievement - Good Article.''' The team were really pleased to find that the <html><a href="http://www.good.is/post/land-grab-could-bioremediation-turn-pacific-garbage-patch-into-habitable-island/" title="Article" target="_blank">Good</a></html> had written an article about our project. </div> | <div class="notebook-achievement"> '''Achievement - Good Article.''' The team were really pleased to find that the <html><a href="http://www.good.is/post/land-grab-could-bioremediation-turn-pacific-garbage-patch-into-habitable-island/" title="Article" target="_blank">Good</a></html> had written an article about our project. </div> | ||
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- | <div class="notebook-wetlab"> '''Wet Lab.''' Today we were keen to test the success for irrE as well as laccase PCR from yesterday. Unfortunately, there were no bands in the 2000bp regions only in the regions of 100bp. We suspected that it was not DNA but RNA. As we had a few unsuccessful transformations previously we decided to test the effectiveness of our antibiotics. All antibiotics (ampicillin, chloramphenicol, tetracyclin, kanamycin)that we had were plated out together with LB agar. We are hoping to see no growth tomorrow to | + | <div class="notebook-wetlab"> '''Wet Lab.''' Today we were keen to test the success for irrE as well as laccase PCR from yesterday. Unfortunately, there were no bands in the 2000bp regions only in the regions of 100bp. We suspected that it was not DNA but RNA. As we had a few unsuccessful transformations previously we decided to test the effectiveness of our antibiotics. All antibiotics (ampicillin, chloramphenicol, tetracyclin, kanamycin)that we had were plated out together with LB agar. We are hoping to see no growth tomorrow to conclude that antibiotics had nothing to do with our unsuccessful transformations.</div> |
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+ | == Reflections == | ||
+ | |||
+ | This week has been an encouraging change to the previous weeks in the lab. We received further training in the lab, in an attempt to eliminate some of the problems we have been experiencing, and to enable us to work more efficiently. We also had our speed debate on Tuesday which was a great success, with an interesting turn-out of people. | ||
+ | |||
+ | <div class="notebook-success"> '''Speed Debate.''' | ||
+ | |||
+ | <div class="notebook-fail"> '''Taq Polymerase.''' | ||
+ | |||
+ | {{:Team:University_College_London/templates/foot}} | ||
== Friday 10th August == | == Friday 10th August == |
Revision as of 09:32, 14 August 2012
Contents |
Notebook: Week 9
Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16
Aims of the Week
The aims of the week include continuing with attempts at ligation in the lab, and the transformation of any remaining BioBricks. On the wiki, we hope to get a more detailed account of our research on the site, and continue with our new Lab book. As far as modelling is concerned, we need to create a more advanced version of the degradation model, and to create a basic model for cell toxicity and salt tolerance. With regard Human Practice, our speed debate is this week, so we want to hear the different opinions from the public regarding the release/use of synthetic organisms to combat pollution, and also explore into the ethical, social and legal issues concerned with this.
Monday 6th August
Tuesday 7th August
Wednesday 8th August
Thursday 9th August
Reflections
This week has been an encouraging change to the previous weeks in the lab. We received further training in the lab, in an attempt to eliminate some of the problems we have been experiencing, and to enable us to work more efficiently. We also had our speed debate on Tuesday which was a great success, with an interesting turn-out of people.