Team:University College London/Notebook/Week8
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<div class="notebook-wetlab"> '''Wet Lab.''' Todays activities included PCR reactions of plasmid backbones, and analysis of their concentration by nanodrop. This proved somewhat disappointing. There was also miniprep of our Tetracycline Repressor (BBa_C0040) and the Gas Vesicle Cluster (BBa_I750016), but both proved to have very low [plasmid concentration. We also grew up some of our W3100 cells transformed with the Salt Tolerance BioBrick (BBa_K398108), as well as some untransformed W3100s, for characterisation of salt tolerance tomorrow.</div> | <div class="notebook-wetlab"> '''Wet Lab.''' Todays activities included PCR reactions of plasmid backbones, and analysis of their concentration by nanodrop. This proved somewhat disappointing. There was also miniprep of our Tetracycline Repressor (BBa_C0040) and the Gas Vesicle Cluster (BBa_I750016), but both proved to have very low [plasmid concentration. We also grew up some of our W3100 cells transformed with the Salt Tolerance BioBrick (BBa_K398108), as well as some untransformed W3100s, for characterisation of salt tolerance tomorrow.</div> | ||
- | <div class="notebook-rathenau"> '''Rathenau.''' Erin finished her write up of the Rathenau debate, and after proof reading from other members of the team sent it to an advisor for assessment. < | + | <div class="notebook-rathenau"> '''Rathenau.''' Erin finished her write up of the Rathenau debate, and after proof reading from other members of the team sent it to an advisor for assessment. </div> |
== Wednesday 1st August == | == Wednesday 1st August == |
Revision as of 09:41, 4 August 2012
Contents |
Notebook: Week 8
Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16
Aims of the Week
The Lab team are aiming to begin assembly of Ribosome Binding Site and Constitutive Promoter, which could then be used for a number of our modules. James and Leonard have also been working towards characterising salt tolerance, and aim to begin doing this by the end of the week. The protocol for this experiment has been decided, and so it is likely we will have some characterisation data by the end of the week. Rhiannon's aim was to finish setting up the wiki LabBook, and publish it on the wiki. The Rathenau Debate Deadline is approaching, and Erin plans to make the final touches before it is sent off. Carina also wants to confirm her plans for the architectural project and begin a diary documenting her designs.
Monday 30th July
Tuesday 31st July
Wednesday 1st August
Thursday 2nd August
Wetlab: James completed the protocol for the salt characterisation, which appears to have been successful. This will be repeated early next week. There was also a gel for the repeat of the PCR of plasmid backbones, which again proved unsuccessful - for reasons that are not clear to us.
Also given the problems we have had with transformation - we have set up another series on transformations, which will attempt to eliminate some of the causes of problems already experienced. We are also allowing only two members of the team to carry out the protocol from start to finish, to limit the number of possible problems. BioBricks that are being transformed are the Constitutive Promoter (BBa_J23119) and Ribosome Binding Site (BBa_B0034) due to their low concentration. Also included is a a spare Constitutive Promoter (BBa_J23100) and a spare Ribosome Binding Site (BBa_B0030)