Revision as of 22:36, 3 October 2012

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Protocols

Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.

General Protocols

Agarose Gel Electrophresis
Bacterial Genomic DNA Purification
Bacterial Transformation
Construction Techniques
Gel Extraction
LB Agar Plates
Overnight Cultures
PCR Purification
Plasmid Purification (from E. coli)
Preparing Chemically Competent Cells (E. coli)
Preparing Glycerol Stocks (E. coli)
Rehydration of Registry DNA
Reviving Freeze-dried Bacterial Cultures from DMSZ
Site-Directed Mutagenesis
Taq PCR Protocol

Electrochemistry Protocols

Cyclic Voltammetry
Potentiostatic Standard Curve Generation
Reporter Expression Detection

Desulfurization Protocols

Decarboxylation Protocols

PetroBrick Validation Assay

Denitrogenation Protocols

Decatecholization Protocols

Decatecholization Assay

Transposon Mutant Library for Toxin Detection

Transposon-Mediated Mutant Library Generation

Kill Switch Protocols

Characterization of mgtA regulation with GFP
Characterization of mgtA regulation with S7 killgene
Nuclease assay

Bioreactor Assays Protocols

Bacterial Growth Experiments

Belt Selection Tests:

Modeling Protocols

Modelling validation experiments

Revision as of 22:36, 3 October 2012 (view source) Yqi2011 (Talk \| contribs) ← Older edit		Revision as of 22:36, 3 October 2012 (view source) Yqi2011 (Talk \| contribs) Newer edit →
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-	<h2>Modeling</h2>	+	<h2>Modeling Protocols</h2>
	<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Modelvalidation">Modelling validation experiments</a></li>		<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Modelvalidation">Modelling validation experiments</a></li>

Team:Calgary/Notebook/Protocols

From 2012.igem.org