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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li> | ||
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dmszfreezedry"> | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dmszfreezedry">Reviving Freeze-dried Bacterial Cultures from DMSZ</a></li> |
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mutagenesis">Site-Directed Mutagenesis</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mutagenesis">Site-Directed Mutagenesis</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/taqpcr">Taq PCR Protocol</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/taqpcr">Taq PCR Protocol</a></li> | ||
Revision as of 21:56, 2 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- Gel Extraction
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DMSZ
- Site-Directed Mutagenesis
- Taq PCR Protocol
Electrochemistry Protocols
- Electorchemistry protocols (include agarose bead making)
Desulfurization
- Desulphuirzation assay protocol
- Catalase assay
- HPAC assay
Decarboxylation
- Petrobrick/oleT assay protocols/ GC MS
Denitrogenation
Decatecholization
- Decatecholization (xylE and incubation with the Petrobrick)
Transposon
Kill Switch
- Nuclease assay
- Magnesium circuit characterization
- Characterization of the mgtA circuit with S7 promoter
Bioreactor
- Bioreactor assays
Modeling
- Modelling validation experiments
