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Team:NYMU-Taipei/ymin3.html
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<div id="ymi_header"> | <div id="ymi_header"> | ||
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| - | <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/ | + | <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/1/15/Ymi_header1.jpg" border="0"></a> |
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<p>NosZ-NorCB<br /> | <p>NosZ-NorCB<br /> | ||
In<em> Pseudomonas aeruginosa PAO1</em>, NorCB is found as two sub-units – nitric oxide reductase subunit C and B. As a matter of fact, these two subunits are adjacent genes. And thus we cloned them together to produce a functional reductase. <div class=out style='text-align:center'> | In<em> Pseudomonas aeruginosa PAO1</em>, NorCB is found as two sub-units – nitric oxide reductase subunit C and B. As a matter of fact, these two subunits are adjacent genes. And thus we cloned them together to produce a functional reductase. <div class=out style='text-align:center'> | ||
| - | <img border="0" src=" | + | <img border="0" src="https://static.igem.org/mediawiki/2012/9/9d/Ymin4.gif" align="center" alt="" /><br /> |
http://www.stanford.edu/group/collman/nor.htm | http://www.stanford.edu/group/collman/nor.htm | ||
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Obviously, the reason we cloned NorCB and NosZ on the same plasmid is to connect the continuous denitrifying procedures together.</p> | Obviously, the reason we cloned NorCB and NosZ on the same plasmid is to connect the continuous denitrifying procedures together.</p> | ||
<div class=out style='text-align:center'> | <div class=out style='text-align:center'> | ||
| - | <img src=" | + | <img src="https://static.igem.org/mediawiki/2012/5/52/Ymin5.gif" alt="" /><br /> |
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<p><a href="http://partsregistry.org/Part:BBa_K896004">http://partsregistry.org/Part:BBa_K896004</a><br /> | <p><a href="http://partsregistry.org/Part:BBa_K896004">http://partsregistry.org/Part:BBa_K896004</a><br /> | ||
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<p>NirN-NirS<br /> | <p>NirN-NirS<br /> | ||
Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.</p> | Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.</p> | ||
| - | <div class="out" style='text-align:center'><img src=" | + | <div class="out" style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/4/42/Ymin6.gif" alt="" width="500" border="0" align="center" /><a href="http://montypython.scs.uiuc.edu/Publications/pubs.php">http://montypython.scs.uiuc.edu/Publications/pubs.php</a><br /> |
<a href="http://partsregistry.org/Part:BBa_K896006">http://partsregistry.org/Part:BBa_K896006</a><br /> | <a href="http://partsregistry.org/Part:BBa_K896006">http://partsregistry.org/Part:BBa_K896006</a><br /> | ||
</div> | </div> | ||
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<p>Nap( Nitrate->Nitrite reductase)<br /> | <p>Nap( Nitrate->Nitrite reductase)<br /> | ||
Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. For instance, even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.</p> | Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. For instance, even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.</p> | ||
| - | <div class="out" style='text-align:center'><img src=" | + | <div class="out" style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/d/d9/Ymin7.gif" alt="" width="399" border="0" align="center" /><br /> |
<p><a href="http://partsregistry.org/Part:BBa_K896007">http://partsregistry.org/Part:BBa_K896007</a><br /> | <p><a href="http://partsregistry.org/Part:BBa_K896007">http://partsregistry.org/Part:BBa_K896007</a><br /> | ||
</p></div> | </p></div> | ||
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<li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymin2.html">Methods</a></li> | <li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymin2.html">Methods</a></li> | ||
<li><a title="Results" href="https://2012.igem.org/Team:NYMU-Taipei/ymin3.html">Results</a></li> | <li><a title="Results" href="https://2012.igem.org/Team:NYMU-Taipei/ymin3.html">Results</a></li> | ||
| - | <li><a title="Practical Application & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymin4.html">Practical Application | + | <li><a title="Practical Application & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymin4.html">Practical Application &<br /> |
| - | References</li> | + | References</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li><a title="Abstract" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq1.html">Abstract</a></li> | <li><a title="Abstract" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq1.html">Abstract</a></li> | ||
<li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li> | <li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li> | ||
| - | <li><a title=" | + | <li><a title="Experiments" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Experiments</a></li> |
| - | <li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html"> | + | <li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results & References</a></li><li><a title="Further Experiments after Asia Jamboree" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq5.html">Further Experiments after Asia Jamboree</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li><a title="Methods & Materials" href="https://2012.igem.org/Team:NYMU-Taipei/ymic3.html">Methods & Materials</a></li> | <li><a title="Methods & Materials" href="https://2012.igem.org/Team:NYMU-Taipei/ymic3.html">Methods & Materials</a></li> | ||
<li><a title="Results & Discussion" href="https://2012.igem.org/Team:NYMU-Taipei/ymic4.html">Results & Discussion</a></li> | <li><a title="Results & Discussion" href="https://2012.igem.org/Team:NYMU-Taipei/ymic4.html">Results & Discussion</a></li> | ||
| - | <li><a title=" | + | <li><a title="Conclusion & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymic5.html">Conclusion & References</a></li> |
</ul> | </ul> | ||
Latest revision as of 01:49, 27 October 2012
Results
Part : BBa_K896004
NosZ-NorCB
In Pseudomonas aeruginosa PAO1, NorCB is found as two sub-units – nitric oxide reductase subunit C and B. As a matter of fact, these two subunits are adjacent genes. And thus we cloned them together to produce a functional reductase.
http://www.stanford.edu/group/collman/nor.htm
NosZ is the structure gene of nitrous reductase. Very few microorganisms process similar enzyme, and many of them are homologs according to NCBI database. Furthermore, it performs the last procedure of bio-denitrification – nitrous oxides to nitrogen – in our model.
Obviously, the reason we cloned NorCB and NosZ on the same plasmid is to connect the continuous denitrifying procedures together.
Part:BBa_K896006
NirN-NirS
Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.
Part:BBa_K896007
Nap( Nitrate->Nitrite reductase)
Nap is periplasmic nitrate reductase, which is known for the ability to reduce nitrate into nitrite. In microorganisms, similar reductases are often observed. For instance, even E. coli has its own nitrate reductase and can use nitrate as final electron acceptor. Moreover, we acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774.
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Denitrifying Machine
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Sulfur Oxide Terminator
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Sulfide as Energy Generator
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Cd+2 Collector
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Becoming Venusian
