Team:University College London/Notebook/Week14

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= Notebook: Week 14 =
= Notebook: Week 14 =
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== Aims of the Week ==
== Aims of the Week ==
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This week we are making a push to upload all of our data to the wiki and stay on track with the Wiki Gantt chart Rhiannon created - Erin and Joanne are focusing on adding our models. Philipp will be going to Manchester to speak to the founders of the Manchester DIYbio space, MadLab.
== Monday 10th September ==
== Monday 10th September ==
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<div class="notebook-meeting"> '''Bielefeld Collaboration''' Aurelija corresponded with Sebastien from iGEM Bielefeld to discuss how we are both modelling our use of laccase.</div>
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<div class="notebook-diybio"> '''DIYBio Exhibition Floorplan ''' - Philipp and Bethan worked on a <html><a href="https://static.igem.org/mediawiki/2012/3/31/Ucl2012_HumanPractices_SynBio_Eventfloorplan.png" title="C-Lab" target="_blank">floor plan</a></html> for the exhibition.</div>  
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<div class="notebook-meeting"> '''Hackspace''' Yeping finished planning the workshops for the collaboration with the London Hackspace. We're really looking forward to the workshops starting on Wednesday! </div>
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<div class="notebook-bielefeld"> '''Bielefeld Collaboration: Laccase Exchange''' - Aurelija decoded the Material Transfer Agreement (MTA) form we have to complete to exchange laccase with Bielefeld. </div>
== Tuesday 11th September ==
== Tuesday 11th September ==
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<div class="notebook-meeting"> '''Darmstadt Collaboration''' Bouran swapped address with Henrik from Darmstadt today, we'll be sending them Laccase for characetrisation, while they are sending us a cutinase enzyme for expression in our Roseobacter.</div>
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<div class="notebook-regional"> '''Regional Jamboree Registration''' - The whole team successfully registered today for iGEM 2012 Regional Jamboree in Europe! </div>
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<div class="notebook-news"> '''Press: Sponsume Article''' - The crowdfundraising website posted a short article Philipp wrote about science fundraising for short-term projects like iGEM: http://www.sponsume.com/blog/university-college-london-igems-experiment-crowdfunding </div>
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<div class="notebook-diybio"> '''DIYbio: MadLab''' - Philipp went to Manchester to speak to the MadLab founders (http://madlab.org.uk/) about DIYbio culture and public outreach.</div>
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== Wednesday 12th September ==
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<div class="notebook-presentation"> '''Practice Presentation''' - Today the team presented to our supervisors and received some good feedback. Overall we really need to improve the clarity of our presentation, it's hard to concisely summarise a summer of work.</div>
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<div class="notebook-meeting"> '''Purdue Collaboration''' James was in touch with Chris from Purdue again to discuss swapping protocols for curli characteristion. </div>
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<div class="notebook-diybio"> '''DIYBio Exhibition ''' - At the exhibition we want to run a gel in the centre of the room. We need a box to safely contain the gel, light and battery without being knocked over. Helping Philipp and Bethan, Howard has created a <html><a href="https://static.igem.org/mediawiki/2012/2/20/UCL2012_HumanPractices_SynBio_Eventboxdesign.png" title="C-Lab" target="_blank">design for the box</a></html> that we will construct with the hackers in the London Hackspace. Bethan has found a podium for our exhibit with the help of Rona from UCL Estates. </div>
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<div class="notebook-meeting"> '''Lab clean-up''' Safety always first. We had a huge clean-up of the lab, including scrubbing the microwave spotless! We're now ready for our last month in the lab. </div>
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<div class="notebook-bielefeld"> '''Bielefeld''' - Bielefeld sent us their laccase! </div>
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== Wednesday 29th August ==
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== Thursday 13th September ==
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<div class="notebook-meeting"> '''British Science Association''' Philipp and Bethan met with Roland Jackson to discuss our DIYbio collaboration. </div>
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<div class="notebook-paris"> '''Paris Visit''' - Philipp corresponded with Gail who booked our flights to Paris! </div>
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<div class="notebook-meeting"> '''Hackspace Day 1''' We went en masse to the London Hackspace this evening for the first workshop. Carina, Yeping, Philipp, Martina and Bethan were all present. Howard met us there. We bought a glycerol stock of W3110 ''E. Coli'' and empty petri dishes to show the biohackers how we make minimal agar plates for day 1 protocol of making competent cells. Quite a few people turned up and they were very keen to have a go!</div>
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<div class="notebook-meeting"> ''iGEM connection''' Bethan is organising with iGEM Paris-Bettencourt a trip to Paris to discuss how our two project approach biosafety, and also discuss aspects of our DIYbio collaboration with members of the CRI's Education X.0 project. Paris iGEM also invited us to participate in their debate on the 16th! </div>
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== Thursday 30th August ==
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<div class="notebook-meeting"> '''Exhibition''' - Philipp secured audio-visual equipment for our Public BioBrick exhibition. </div>
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<div class="notebook-meeting"> '''Hackspace Day 2''' Together with the hackers, Bethan helped to build a shaker to agitate our cells overnight. This involved using a number of equipment only available at the Hackspace, including hand saw, glue gun and motor. The biohackers were very keen to give us an electronics tutorial as we build our circuit for the shaker. A variable resistor was included in the circuit design so we can control the speed of the shaker.</div>
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<div class="">We also performed PCR of the cholorophenicol plasmid backbone required for our ligations. At hackspace, they have a very cool 'ancient' thermocycler. It has no heated lid so mineral oil has to be added to samples to prevent evaporation. The biohackers showed us how to program the machine. There are so many steps compared to the one used in our lab!</div>
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<div class="notebook-modelling"> '''Wiki meeting''' Erin, Joanne, and Aurelija meet to finalise our modelling plans. As of yet none of our modelling is up on the wiki, so we agree on a series of deadlines to ensure everything is written up well in time for the wiki freeze. </div>
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== Friday 31st August ==
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== Friday 14th September ==
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<div class="notebook-meeting"> '''Hackspace Day 3''' Our shaker from yesterday was still alive today and the liquid cultures in falcon tubes are all cloudy showing growth! Together with the biohackers we ran a 1% gel of the PCR product from yesterday using their DIY gel tray. Their tray didn't have a cover, you can also see how the wires are connected!  Using a hand held UV filter and UV light, we visualized our gel. The PCR has worked! Gel shows the expected band at 2070bp. PCR clean-up is to be done at the UCL workshops since the centrifuge at Hackspace is only estimated to be able to do 5000rpm </div>
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<div class="notebook-hackspace"> '''Hackspace collaboration: Evaluation''' Philipp scheduled interviews with all the UCL and Hackspace workshop participants to evaluate the collaboration and workshops. </div>
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<div class="notebook-meeting"> '''Press''' C-lab published an <html><a href="http://c-lab.co.uk/blogs/visiting-london-hackspace-with-ucl-igem.html" title="C-Lab" target="_blank">exciting write-up</a></html> of our 1st day at the Hackspace </div>
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<div class="notebook-brainstorm"> '''Meeting of Young Minds Video Opener''' - Philipp, Erin and Leonard brainstormed the style of video to open our debate at the Meeting of Young Minds. They settled on an advertisement. </div>
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Latest revision as of 21:42, 26 September 2012

Contents

Notebook: Week 14

Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16

loading facebook photos...

Aims of the Week

This week we are making a push to upload all of our data to the wiki and stay on track with the Wiki Gantt chart Rhiannon created - Erin and Joanne are focusing on adding our models. Philipp will be going to Manchester to speak to the founders of the Manchester DIYbio space, MadLab.

Monday 10th September

DIYBio Exhibition Floorplan - Philipp and Bethan worked on a floor plan for the exhibition.
Bielefeld Collaboration: Laccase Exchange - Aurelija decoded the Material Transfer Agreement (MTA) form we have to complete to exchange laccase with Bielefeld.

Tuesday 11th September

Regional Jamboree Registration - The whole team successfully registered today for iGEM 2012 Regional Jamboree in Europe!
Press: Sponsume Article - The crowdfundraising website posted a short article Philipp wrote about science fundraising for short-term projects like iGEM: http://www.sponsume.com/blog/university-college-london-igems-experiment-crowdfunding
DIYbio: MadLab - Philipp went to Manchester to speak to the MadLab founders (http://madlab.org.uk/) about DIYbio culture and public outreach.

Wednesday 12th September

Practice Presentation - Today the team presented to our supervisors and received some good feedback. Overall we really need to improve the clarity of our presentation, it's hard to concisely summarise a summer of work.
DIYBio Exhibition - At the exhibition we want to run a gel in the centre of the room. We need a box to safely contain the gel, light and battery without being knocked over. Helping Philipp and Bethan, Howard has created a design for the box that we will construct with the hackers in the London Hackspace. Bethan has found a podium for our exhibit with the help of Rona from UCL Estates.
Bielefeld - Bielefeld sent us their laccase!

Thursday 13th September

Paris Visit - Philipp corresponded with Gail who booked our flights to Paris!
Exhibition - Philipp secured audio-visual equipment for our Public BioBrick exhibition.
Wiki meeting Erin, Joanne, and Aurelija meet to finalise our modelling plans. As of yet none of our modelling is up on the wiki, so we agree on a series of deadlines to ensure everything is written up well in time for the wiki freeze.

Friday 14th September

Hackspace collaboration: Evaluation Philipp scheduled interviews with all the UCL and Hackspace workshop participants to evaluate the collaboration and workshops.
Meeting of Young Minds Video Opener - Philipp, Erin and Leonard brainstormed the style of video to open our debate at the Meeting of Young Minds. They settled on an advertisement.

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