Team:Valencia Biocampus/talking

From 2012.igem.org

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Our aim in this part of the project is to detect when the yeast starts to ferment. To do this, we are going to use two gene constructions. The first construction consists of the ADH2 promoter fused to the YAP1 protein coding sequence. In the second construction the TRR promoter is fused to the GFP (Green Fluorescence Protein) coding sequence.
Our aim in this part of the project is to detect when the yeast starts to ferment. To do this, we are going to use two gene constructions. The first construction consists of the ADH2 promoter fused to the YAP1 protein coding sequence. In the second construction the TRR promoter is fused to the GFP (Green Fluorescence Protein) coding sequence.
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We use the ADH2 promoter (alcohol dehydrogenase) to sense the glucose/ethanol concentration. When this promoter is activated, YAP1 is expressed. This protein will attach to TRR promoter (thioredoxin reductase), which will become activated if the constitutive yeast protein SKN7 is in its oxidized form and also interacts with the promoter. This activation will enable transcription of GFP. H2O2 has to be added in order to oxidize the SKN7 protein.
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<ul>
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        <li>The basic life cycle of our biological agent is based on an input/output process through the use of interfaces.
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        <li>The input used is a voice signal (question), which will be collected by our voice recognizer
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        <li>The voice recognizer identifies the question and, through the program in charge of establishing the communication, its corresponding identifier is written in the
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              assigned port of the arduino.
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        <li>The (sw=software?) of the arduino reads the written identifier and, according to it, the corresponding port is selected, indicating the flourimeter which wavelength has
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              to be emitted on the culture.  There are four possible questions (q), and each of them is associated to a different wavelength.  
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        <li>The fluorimeter emits light (Bioinput), exciting the compound through optic filters.
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        <li>Due to the excitation produced, the compound emits fluorescence (BioOutput), which is measured by the fluorimeter with a sensor.
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        <li>This fluorescence corresponds to one of the four possible answers (r: response)
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        <li>The program of the arduino identifies the answer and writes its identifier in the corresponding port.
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        <li>The communication program reads the identifier of the answer from the port.
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        <li>Julius emits the answer via a voice signal. (Output)
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</ul>
</div>
</div>

Revision as of 15:47, 30 August 2012



Talking Interfaces


THE PROCESS




Our aim in this part of the project is to detect when the yeast starts to ferment. To do this, we are going to use two gene constructions. The first construction consists of the ADH2 promoter fused to the YAP1 protein coding sequence. In the second construction the TRR promoter is fused to the GFP (Green Fluorescence Protein) coding sequence.

  • The basic life cycle of our biological agent is based on an input/output process through the use of interfaces.
  • The input used is a voice signal (question), which will be collected by our voice recognizer
  • The voice recognizer identifies the question and, through the program in charge of establishing the communication, its corresponding identifier is written in the assigned port of the arduino.
  • The (sw=software?) of the arduino reads the written identifier and, according to it, the corresponding port is selected, indicating the flourimeter which wavelength has to be emitted on the culture. There are four possible questions (q), and each of them is associated to a different wavelength.
  • The fluorimeter emits light (Bioinput), exciting the compound through optic filters.
  • Due to the excitation produced, the compound emits fluorescence (BioOutput), which is measured by the fluorimeter with a sensor.
  • This fluorescence corresponds to one of the four possible answers (r: response)
  • The program of the arduino identifies the answer and writes its identifier in the corresponding port.
  • The communication program reads the identifier of the answer from the port.
  • Julius emits the answer via a voice signal. (Output)
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