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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Carbazole GC-MS Analysis">GC-MS Analysis</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Carbazole GC-MS Analysis">GC-MS Analysis</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gelextraction">Gel Extraction</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gelextraction">Gel Extraction</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Gibson Assembly">Gibson Assembly</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media">M9 Minimal Media Preparation</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media">M9 Minimal Media Preparation</a></li> | ||
| Line 26: | Line 27: | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mutagenesis">Site-Directed Mutagenesis</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mutagenesis">Site-Directed Mutagenesis</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/soe">Splice Overlap Extension PCR (SOE PCR)</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/soe">Splice Overlap Extension PCR (SOE PCR)</a></li> | ||
| - | + | ||
| Line 45: | Line 46: | ||
<h2>Denitrogenation Protocols</h2> | <h2>Denitrogenation Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay"><i>AmdA</i> Characterization Assay</a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/carbazole">Carbazole Degradation Assay</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/carbazole">Carbazole Degradation Assay</a></li> | ||
| + | |||
<h2>Decatecholization Protocols</h2> | <h2>Decatecholization Protocols</h2> | ||
| Line 55: | Line 58: | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">Characterization of mgtA regulation with GFP </a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">Characterization of mgtA regulation with GFP </a></li> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit">Characterization of mgtA regulation with S7 killgene </a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit">Characterization of mgtA regulation with S7 killgene </a></li> | ||
| - | |||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Prha Characterization">Characterization of <i>P<sub>rha</sub></i> with GFP</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Prha Characterization">Characterization of <i>P<sub>rha</sub></i> with GFP</a></li> | ||
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/GlycineAssays">Glycine Auxotrophic Assays (Glycine Media Test, Petrobrick Test | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/escapersassay">Kill Assay for rhamnose system</a></li> |
| + | </a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/GlycineAssays">Glycine Auxotrophic Assays (Glycine Media Test, and Petrobrick Test) | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/nucleaseassay">Nuclease assay</a></li> | ||
| + | </a></li> | ||
<h2>Bioreactor Assays Protocols</h2> | <h2>Bioreactor Assays Protocols</h2> | ||
Latest revision as of 03:54, 27 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- GC-MS Analysis
- Gel Extraction
- Gibson Assembly
- LB Agar Plates
- M9 Minimal Media Preparation
- Organic Extraction
- Overnight Cultures
- PCR Purification
- PicoGreen Assay
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DSMZ
- Site-Directed Mutagenesis
- Splice Overlap Extension PCR (SOE PCR)
Electrochemistry Protocols
- Cyclic Voltammetry
- Potentiostatic Standard Curve Generation
- Reporter Expression Detection
Desulfurization Protocols
- Catalase assay
- Desulfurization Assay
- HpaC assay
Decarboxylation Protocols
- PetroBrick Validation Assay
- oleT Validation Assay
Denitrogenation Protocols
- AmdA Characterization Assay
- Carbazole Degradation Assay
Decatecholization Protocols
- Decatecholization Assay
Transposon Mutant Library for Toxin Detection
- Transposon-Mediated Mutant Library Generation
Kill Switch Protocols
- Characterization of mgtA regulation with GFP
- Characterization of mgtA regulation with S7 killgene
- Characterization of Prha with GFP
- Kill Assay for rhamnose system
- Glycine Auxotrophic Assays (Glycine Media Test, and Petrobrick Test)
- Nuclease assay
- Bacterial Growth Experiments
- Bacteria Test
- Hydrocarbon Separation Test
- Hydrocarbon Test
- NA and Hexadecane Belt Skim Test
- Tailings Test
- Modelling validation experiments
General Protocols
Bioreactor Assays Protocols
Belt Selection Tests:
