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Team:Calgary/Notebook/Protocols
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<p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> | <p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> | ||
<ul> | <ul> | ||
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<h2>General Protocols</h2> | <h2>General Protocols</h2> | ||
| - | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/agarosegel">Agarose Gel Electrophresis</a></li> | |
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/ | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gemomicprep">Bacterial Genomic DNA Purification</a></li> |
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/ | + | |
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<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/transformation">Bacterial Transformation</a></li> | ||
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/construction">Construction Techniques</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Carbazole GC-MS Analysis">GC-MS Analysis</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/gelextraction">Gel Extraction</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Gibson Assembly">Gibson Assembly</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/lbagar">LB Agar Plates</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media">M9 Minimal Media Preparation</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oextraction">Organic Extraction</a></li> |
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/onculture">Overnight Cultures</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/pcrpurification">PCR Purification</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/picogreen">PicoGreen Assay</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/plasmidminiprep">Plasmid Purification (from <i>E. coli</i>)</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/compcells">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/glycerolstock">Preparing Glycerol Stocks (<i>E. coli</i>) | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dnarehydration">Rehydration of Registry DNA</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/dmszfreezedry">Reviving Freeze-dried Bacterial Cultures from DSMZ</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mutagenesis">Site-Directed Mutagenesis</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/soe">Splice Overlap Extension PCR (SOE PCR)</a></li> | ||
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| + | <h2>Electrochemistry Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/cvs">Cyclic Voltammetry</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/potstd">Potentiostatic Standard Curve Generation</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/potentiostatic">Reporter Expression Detection</a></li> | ||
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| + | <h2>Desulfurization Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/catalase">Catalase assay</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/desulfur">Desulfurization Assay</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hpac">HpaC assay</a></li> | ||
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| + | <h2>Decarboxylation Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/PetroBrick Validation Assay">PetroBrick Validation Assay </a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/oleT in Validation Assay">oleT Validation Assay </a></li> | ||
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| + | <h2>Denitrogenation Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay"><i>AmdA</i> Characterization Assay</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/carbazole">Carbazole Degradation Assay</a></li> | ||
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| + | <h2>Decatecholization Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization Assay</a></li> | ||
| + | <h2>Transposon Mutant Library for Toxin Detection</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li> | ||
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| + | <h2>Kill Switch Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgcircuit">Characterization of mgtA regulation with GFP </a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit">Characterization of mgtA regulation with S7 killgene </a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Prha Characterization">Characterization of <i>P<sub>rha</sub></i> with GFP</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/escapersassay">Kill Assay for rhamnose system</a></li> | ||
| + | </a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/GlycineAssays">Glycine Auxotrophic Assays (Glycine Media Test, and Petrobrick Test) | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/nucleaseassay">Nuclease assay</a></li> | ||
| + | </a></li> | ||
| + | |||
| + | <h2>Bioreactor Assays Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/bacgrowth">Bacterial Growth Experiments</a></li> | ||
| + | <p>Belt Selection Tests:</p> | ||
| + | <ul> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/bactest">Bacteria Test</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hcseptest">Hydrocarbon Separation Test</a></li><li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hctest">Hydrocarbon Test</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/hdbeltskim">NA and Hexadecane Belt Skim Test</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tailingtest">Tailings Test</a></li> | ||
| + | </ul> | ||
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| + | <h2>Modeling Protocols</h2> | ||
| + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/Modelvalidation">Modelling validation experiments</a></li> | ||
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</ul> | </ul> | ||
Latest revision as of 03:54, 27 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- GC-MS Analysis
- Gel Extraction
- Gibson Assembly
- LB Agar Plates
- M9 Minimal Media Preparation
- Organic Extraction
- Overnight Cultures
- PCR Purification
- PicoGreen Assay
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DSMZ
- Site-Directed Mutagenesis
- Splice Overlap Extension PCR (SOE PCR)
Electrochemistry Protocols
- Cyclic Voltammetry
- Potentiostatic Standard Curve Generation
- Reporter Expression Detection
Desulfurization Protocols
- Catalase assay
- Desulfurization Assay
- HpaC assay
Decarboxylation Protocols
- PetroBrick Validation Assay
- oleT Validation Assay
Denitrogenation Protocols
- AmdA Characterization Assay
- Carbazole Degradation Assay
Decatecholization Protocols
- Decatecholization Assay
Transposon Mutant Library for Toxin Detection
- Transposon-Mediated Mutant Library Generation
Kill Switch Protocols
- Characterization of mgtA regulation with GFP
- Characterization of mgtA regulation with S7 killgene
- Characterization of Prha with GFP
- Kill Assay for rhamnose system
- Glycine Auxotrophic Assays (Glycine Media Test, and Petrobrick Test)
- Nuclease assay
- Bacterial Growth Experiments
- Bacteria Test
- Hydrocarbon Separation Test
- Hydrocarbon Test
- NA and Hexadecane Belt Skim Test
- Tailings Test
- Modelling validation experiments
General Protocols
Bioreactor Assays Protocols
Belt Selection Tests:
