Team:NYMU-Taipei/ymiq1.html

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<div class="title">Abstract</div>
<div class="title">Abstract</div>
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   <p><span class="subtitle">Resistance of Cyanobacteria to Sulfide compound --&gt; Sulfide-Quinone Reductase</span></p>
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   <p><span class="subtitle">Hydrogen Sulfide --&gt; A Novel Energy Currency for Photosynthesis</span></p>
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   <p>Several Cyanobacteria have Sulfide-Quinone Reductase (SQR) and thus the ability to deprive electron from sulfide compound. According to both databases of NCBI and KEGG, the sqr in Synechococcus SP. PCC 7002 shared great similarity with that of Oscillatoria limnetica, which is reported to exhibit anoxygenic photosynthesis by consumed sulfide anion. Since we planned to express sqr from Synechococcus SP. PCC 7002 in Synechococcus SP. PCC 7942 and Escherichia coli, the experiment was designed to testify the property of the sqr. DCMU was added in the medium to inhibit photosystem II, and therefore only sodium sulfide in the medium can provide electron for carbon photoassimilation. By creating different dilution of sodium sulfide, we expected that the more sodium sulfide was present, the better the cell grew. <br />
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   <p> Besides removing sulfur oxides, another great contribution of these enzymes mentioned above is storing energy inside the chemical bond of hydrogen sulfide. Sulfide-quinone reductase, or sqr, then comes into effect. Sqr is able to oxidize sulfide compounds into sulfur and release the bond energy for further favorable reaction, including nitrogen removal and carbon fixation.
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In photoassimilation, sqr would replace the function of photosystem II. For ordinary photosynthesis, photosystem II disassociates water and oxygen is formed subsequently. In the case of our engineered cyanobacteria, hydrogen sulfide (H2S) is oxidized and sulfur is produced. In other words, we constructed sulfur version of photosynthesis instead of traditional oxygen and water version.  
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                 <li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li>
                 <li><a title="Methods" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li>
                 <li><a title="Experiments" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Experiments</a></li>
                 <li><a title="Experiments" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Experiments</a></li>
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                 <li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results &amp; References</a></li>
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                 <li><a title="Results & References" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results &amp; References</a></li><li><a title="Further Experiments after Asia Jamboree" href="https://2012.igem.org/Team:NYMU-Taipei/ymiq5.html">Further Experiments after Asia Jamboree</a></li>
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Latest revision as of 02:36, 27 October 2012

NYMU iGEM

Abstract

Hydrogen Sulfide --> A Novel Energy Currency for Photosynthesis

Besides removing sulfur oxides, another great contribution of these enzymes mentioned above is storing energy inside the chemical bond of hydrogen sulfide. Sulfide-quinone reductase, or sqr, then comes into effect. Sqr is able to oxidize sulfide compounds into sulfur and release the bond energy for further favorable reaction, including nitrogen removal and carbon fixation. In photoassimilation, sqr would replace the function of photosystem II. For ordinary photosynthesis, photosystem II disassociates water and oxygen is formed subsequently. In the case of our engineered cyanobacteria, hydrogen sulfide (H2S) is oxidized and sulfur is produced. In other words, we constructed sulfur version of photosynthesis instead of traditional oxygen and water version.


The special ”CO2” infused device for cyanobacteria incubation

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