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Team:Calgary/Notebook/Protocols/mgcircuit
From 2012.igem.org
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{{Team:Calgary/TemplateNotebookOrange| | {{Team:Calgary/TemplateNotebookOrange| | ||
| - | TITLE=Characterization of | + | TITLE=Characterization of mgtA regulation with GFP| |
CONTENT=<html> | CONTENT=<html> | ||
<ol> | <ol> | ||
<li>Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl<font style="text-transform: lowercase;">2</font>.</li> | <li>Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl<font style="text-transform: lowercase;">2</font>.</li> | ||
| - | <li>mgtAp-rb-K082003</li> | + | <ul><li>mgtAp-rb-K082003</li> |
<li>mgtAp-rb</li> | <li>mgtAp-rb</li> | ||
<li>R0040-mgtArb-K082003</li> | <li>R0040-mgtArb-K082003</li> | ||
| - | <li>The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl<font style="text-transform: lowercase;"> | + | <li>R0040-B0034-K082003</li></ul> |
| + | <li>The next morning the cells were spun down and washed with <a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media">M9 minimal media</a> and appropriate amounts of MgCl | ||
| + | <ul><li> 0mM MgCl<sub>2</sub></li> | ||
| + | <li> 0.01mM MgCl<sub>2</sub></li> | ||
| + | <li> 0.1mM MgCl<sub>2</sub></li> | ||
| + | <li> 1mM MgCl<sub>2</sub></li> | ||
| + | <li> 10mM MgCl<sub>2</sub></li></ul> <font style="text-transform: lowercase;"></font></li> | ||
| + | |||
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.</li> | <li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.</li> | ||
</ol> | </ol> | ||
Latest revision as of 02:16, 4 October 2012
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Characterization of mgtA regulation with GFP
- Cells containing the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- mgtAp-rb-K082003
- mgtAp-rb
- R0040-mgtArb-K082003
- R0040-B0034-K082003
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl
- 0mM MgCl2
- 0.01mM MgCl2
- 0.1mM MgCl2
- 1mM MgCl2
- 10mM MgCl2
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and florescence was measured every hour for 24 hours at 475nm excitation and 515nm emission.
