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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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<h2>Decatecholization</h2> | <h2>Decatecholization</h2> | ||
| - | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/decatecholization">Decatecholization Assay</a></li> |
<h2>Transposon Mutant Library for Toxin Detection</h2> | <h2>Transposon Mutant Library for Toxin Detection</h2> | ||
<li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li> | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/tnscreen">Transposon-Mediated Mutant Library Generation</a></li> | ||
Revision as of 22:23, 3 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Agarose Gel Electrophresis
- Bacterial Genomic DNA Purification
- Bacterial Transformation
- Construction Techniques
- Gel Extraction
- LB Agar Plates
- Overnight Cultures
- PCR Purification
- Plasmid Purification (from E. coli)
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
- Rehydration of Registry DNA
- Reviving Freeze-dried Bacterial Cultures from DMSZ
- Site-Directed Mutagenesis
- Taq PCR Protocol
Electrochemistry Protocols
- Cyclic Voltammetry
- Potentiostatic Standard Curve Generation
- Reporter Expression Detection
Desulfurization
- Desulfurization Assay
- Catalase assay
- HpaC assay
Decarboxylation
- PetroBrick Validation Assay
Denitrogenation
- Organic Extraction
- Carbazole Degradation Assay
- GC-MS Analysis
Decatecholization
- Decatecholization Assay
Transposon Mutant Library for Toxin Detection
- Transposon-Mediated Mutant Library Generation
Kill Switch
- Nuclease assay
- Characterization of mgtA regulation with GFP
- Characterization of the mgtA regulation with S7 killgene
Bioreactor Assays
- Bacterial Growth Experiments
Belt Selection Tests:
- Hydrocarbon Test
- Tailings Test
- Bacteria Test
- Hydrocarbon Separation Test
- NA and Hexadecane Belt Skim Test
Modeling
- Modelling validation experiments
