Team:Calgary/Project/DataPage
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Rather than just sensing toxins in the tailings ponds, our major objective were to detoxify the tailings through the reduction of toxins such as carboxylic acids (mainly naphthenic acids) and catechol, turning them into useable hydrocarbons. Purification of these hydrocarbons would contribute to an added economic and industrial benefit. In order to house this system, we also aimed to design a bioreactor for our bacteria as well as optimize product output through a flux-variability based model. Finally, in order to create higher quality hydrocarbons, we explored desulphurization and denitrogenation pathways in order to upgrade our fuel. To do this in a safe and environmentally sound way, we designed not only structural containment mechanisms, but also genetic containment mechanisms through novel inducible ribo-killswitches.]]<html> | Rather than just sensing toxins in the tailings ponds, our major objective were to detoxify the tailings through the reduction of toxins such as carboxylic acids (mainly naphthenic acids) and catechol, turning them into useable hydrocarbons. Purification of these hydrocarbons would contribute to an added economic and industrial benefit. In order to house this system, we also aimed to design a bioreactor for our bacteria as well as optimize product output through a flux-variability based model. Finally, in order to create higher quality hydrocarbons, we explored desulphurization and denitrogenation pathways in order to upgrade our fuel. To do this in a safe and environmentally sound way, we designed not only structural containment mechanisms, but also genetic containment mechanisms through novel inducible ribo-killswitches.]]<html> | ||
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<h2>Characterization of new parts submitted to the Registry</h2> | <h2>Characterization of new parts submitted to the Registry</h2> |
Revision as of 20:08, 3 October 2012
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Detect and Destroy: Data Page
Design
Characterization of new parts submitted to the Registry
BBa_K902000 and BBa_K902004, : two novel hydrolase enzymes were submitted to the registry for the hydrolysis of two different sugar conjugated electroactive compounds. Used in conjunction with the existing lacZ part in the registry (BBa_I732005,), this allows for the electrochemical detection of three compounds with a single electrode. A uidA inducible generator (BBa_k902002,) was submitted and characterized electrochemically. This data can be found on our electroreporting page.
BBa_K902008,BBa_K902023 and BBa_K902074: three novel riboswitchs were submtted along with two associated promoters (BBa_K902008) and BBa_K902008) and an inducible/repressible promoter BBa_K902065) were submitted to the registry. One of these riboswitches (BBa_K902008) was tested with GFP using this construct, (BBa_K902014), with its promoter and GFP using this construct (BBa_K902017) and with its promoter and the S7 kill gene using this construct BBa_K902018). This data can be found on our killswitch (regulation page.
Genes for denitorgenation and desulphurization were biobricked and submitted. A novel oxidoreductase part BBa_K902058 was also submitted and characterized for use in the desulphurization project. This data can be found on our uprading desulfurization page.
Further characterization of parts already present within the registry
BBa_K590025 Decatecholization - Optimized an existing biobrick for the production of xylE (catechol dioxygenase) for the degradation of catechol using a constitutive promoter. Other OSCAR parts?!?!?!?
Parts Already In the Registry
Optimized the Petrobrick (BBa_590025) to be able to convert naphthenic acids into alkane and alkene compounds.
Additional Work and Characterizations
Developed a program using MATLAB for the optimization of metabolic pathways in synthetic biology metabolic networks. The program allows you to build an artificial synthetic biology network in E. coli and predicts substrates that should be fed to the organism to increase production of the compound. This was characterized and validated in the wetlab with the Petrobrick.
Developed hardware and software for the development of a biosensor using an electrochemical sensor. The software is available on our wiki. (Robert can you help me flush this out a bit, or one of the engineers?